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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-3-27
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pubmed:databankReference |
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pubmed:abstractText |
The first crystal structure of a native di-iron center in an iron-storage protein (bacterio)ferritin is reported. The protein, isolated from the anaerobic bacterium Desulfovibrio desulfuricans, has the unique property of having Fe-coproporphyrin III as its heme cofactor. The three-dimensional structure of this bacterioferritin was determined in three distinct catalytic/redox states by X-ray crystallography (at 1.95, 2.05 and 2.35 A resolution), corresponding to different intermediates of the di-iron ferroxidase site. Conformational changes associated with these intermediates support the idea of a route for iron entry into the protein shell through a pore that passes through the di-iron center. Molecular surface and electrostatic potential calculations also suggest the presence of another ion channel, distant from the channels at the three- and four-fold axes proposed as points of entry for the iron atoms.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1072-8368
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
285-90
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12627224-Bacterial Proteins,
pubmed-meshheading:12627224-Binding Sites,
pubmed-meshheading:12627224-Ceruloplasmin,
pubmed-meshheading:12627224-Crystallography, X-Ray,
pubmed-meshheading:12627224-Cytochrome b Group,
pubmed-meshheading:12627224-Desulfovibrio,
pubmed-meshheading:12627224-Ferritins,
pubmed-meshheading:12627224-Heme,
pubmed-meshheading:12627224-Ion Channels,
pubmed-meshheading:12627224-Iron,
pubmed-meshheading:12627224-Models, Molecular,
pubmed-meshheading:12627224-Oxidation-Reduction,
pubmed-meshheading:12627224-Protein Conformation,
pubmed-meshheading:12627224-Protein Structure, Quaternary,
pubmed-meshheading:12627224-Protein Subunits,
pubmed-meshheading:12627224-Static Electricity
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pubmed:year |
2003
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pubmed:articleTitle |
The nature of the di-iron site in the bacterioferritin from Desulfovibrio desulfuricans.
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pubmed:affiliation |
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, EAN, Apartado 127, 2781-901 Oeiras, Portugal.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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