Linked Life Data

12586707

Source:http://linkedlifedata.com/resource/pubmed/id/12586707

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-2-14
pubmed:abstractText
With the completion of the nucleotide sequences of several complex eukaryotic genomes, tens of thousands of genes have been predicted. However, this information has to be correlated with the functions of those genes to enhance our understanding of biology and to improve human health care. The Drosophila transposon P-element-induced mutations are very useful for directly connecting gene products to their biological function. We designed an efficient transposon P-element-mediated gene disruption procedure and performed genetic screening for single P-element insertion mutations, enabling us to recover 2500 lethal mutations. Among these, 2355 are second chromosome mutations. Sequences flanking >2300 insertions that identify 850 different genes or ESTs (783 genes on the second chromosome and 67 genes on the third chromosome) have been determined. Among these, 455 correspond to genes for which no lethal mutation has yet been reported. The Drosophila genome is thought to contain approximately 3600 vital genes; 1400 are localized on the second chromosome. Our mutation collection represents approximately 56% of the second chromosome vital genes and approximately 24% of the total vital Drosophila genes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-10471706, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-10731132, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-11130711, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-11237011, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-11742088, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-2492966, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-2499512, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-2558049, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-2835286, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-3686007, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-6479445, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-6776413, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-7479892, http://linkedlifedata.com/resource/pubmed/commentcorrection/12586707-8978055
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
195-201
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
A P-element insertion screen identified mutations in 455 novel essential genes in Drosophila.
pubmed:affiliation
The Laboratory of Immunobiology, National Institutes of Health, National Cancer Institute, Frederick, Maryland 21702-1201, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.