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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6C
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pubmed:dateCreated |
2003-1-29
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pubmed:abstractText |
GAPDH, beta-actin and 18S rRNA are widely employed as internal control genes, with the assumption that they are expressed constitutively to similar degrees in different cells and tissues and under different experimental conditions. In this study, we tested this assumption by assessment of the transcription of these three genes in human colonic tissues using a quantitative RT-PCR.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0250-7005
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4173-8
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:12553051-Actins,
pubmed-meshheading:12553051-Adenocarcinoma,
pubmed-meshheading:12553051-Adenoma,
pubmed-meshheading:12553051-Colon,
pubmed-meshheading:12553051-Colonic Neoplasms,
pubmed-meshheading:12553051-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:12553051-Glyceraldehyde-3-Phosphate Dehydrogenases,
pubmed-meshheading:12553051-HT29 Cells,
pubmed-meshheading:12553051-Humans,
pubmed-meshheading:12553051-Intestinal Mucosa,
pubmed-meshheading:12553051-RNA, Messenger,
pubmed-meshheading:12553051-RNA, Ribosomal, 18S,
pubmed-meshheading:12553051-Transcription, Genetic,
pubmed-meshheading:12553051-Tumor Cells, Cultured
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pubmed:articleTitle |
Selection of an internal control gene for quantitation of mRNA in colonic tissues.
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pubmed:affiliation |
Department of Clinical Laboratory Medicine and Division of Laboratory Diagnosis, Sapporo Medical University, School of Medicine, Sapporo, Japan.
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pubmed:publicationType |
Journal Article
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