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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0109317,
umls-concept:C0185117,
umls-concept:C0205107,
umls-concept:C0552639,
umls-concept:C0752312,
umls-concept:C1150579,
umls-concept:C1314939,
umls-concept:C1333340,
umls-concept:C1337092,
umls-concept:C1366882,
umls-concept:C1370600,
umls-concept:C1522558,
umls-concept:C1704259,
umls-concept:C1705079,
umls-concept:C1705767,
umls-concept:C1705791,
umls-concept:C1705987,
umls-concept:C2911684
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pubmed:issue |
5
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pubmed:dateCreated |
2003-4-4
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pubmed:abstractText |
Persistent proteinuria has been indicated to be a major risk factor for the development of tubulointerstitial damage through a process of proinflammatory molecule expression. Monocyte chemoattractant protein-1 (MCP-1) was shown to contribute to recruitment of immune cells into the renal interstitium in acute and chronic renal diseases. However, the molecular mechanisms by which proteinuria causes MCP-1 expression in proximal tubular cells have not been fully clarified. In this study, we examined whether albumin overload-induced MCP-1 expression was regulated by mitogen-activated protein kinase (MAPK) in mouse proximal tubular (mProx) cells. Exposure of mProx cells to delipidated bovine serum albumin (BSA) induced mRNA and protein expression of MCP-1 in a time- and dose-dependent manner. BSA activated extracellular signal-regulated kinase (ERK1/2) and p38 MAPK. The MEK inhibitor U-0126 partially suppressed BSA-induced MCP-1 expression and MCP-1 promoter/luciferase reporter activity. U-0126 also inhibited an increase in nuclear factor-kappaB and activator protein-1 DNA-binding activity of MCP-1 promoter by protein overload in mProx cells. In addition, we found that U-0126 inhibited BSA-induced nuclear factor-kappaB reporter activity and inhibitory protein degradation in mProx cells. In conclusion, these findings indicate that ERK signaling is involved in BSA-induced MCP-1 expression in mProx cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Bovine,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1931-857X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
284
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
F1037-45
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pubmed:dateRevised |
2011-4-28
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pubmed:meshHeading |
pubmed-meshheading:12517735-Animals,
pubmed-meshheading:12517735-Cell Transformation, Viral,
pubmed-meshheading:12517735-Cells, Cultured,
pubmed-meshheading:12517735-Chemokine CCL2,
pubmed-meshheading:12517735-DNA,
pubmed-meshheading:12517735-Enzyme Activation,
pubmed-meshheading:12517735-I-kappa B Proteins,
pubmed-meshheading:12517735-Kidney Tubules, Proximal,
pubmed-meshheading:12517735-Mice,
pubmed-meshheading:12517735-Mice, Inbred C57BL,
pubmed-meshheading:12517735-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12517735-NF-kappa B,
pubmed-meshheading:12517735-Protein Isoforms,
pubmed-meshheading:12517735-RNA, Messenger,
pubmed-meshheading:12517735-Serum Albumin, Bovine,
pubmed-meshheading:12517735-Transcription Factor AP-1
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pubmed:year |
2003
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pubmed:articleTitle |
Involvement of ERK pathway in albumin-induced MCP-1 expression in mouse proximal tubular cells.
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pubmed:affiliation |
Department of Medicine, Shiga University of Medical Science, Shiga 520-2192, Japan.
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pubmed:publicationType |
Journal Article
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