Linked Life Data

12504095

Source:http://linkedlifedata.com/resource/pubmed/id/12504095

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-12-30
pubmed:abstractText
Extravillous trophoblast (EVT) cells of the human placenta proliferate, migrate, and invade the pregnant uterus and its vasculature in order to nourish the fetus. However, the normal EVT cell proliferation, migration, and invasiveness are exquisitely controlled in situ by decidua-produced transforming growth factor-beta (TGF-beta), whereas EVT cancer (choriocarcinoma) cells are TGF-beta-resistant. We found that these cells lack in expression of Smad3, a key transcription factor involved in TGF-beta signaling pathway. To test whether Smad3 restitution restores TGF-beta response in choriocarcinoma cells, we produced a Smad3-expressing cell line (JAR-smad3/c). Since anti-invasive effect of TGF-beta in the normal EVT cells was partly mediated by an upregulation of tissue inhibitor of metalloprotease (TIMP)-1, we examined whether Smad3-restituted JAR cells have restored TGF-beta response of TIMP-1 upregulation. The expression of TIMP-1 mRNA was found to be low in JAR and JAR-smad3/c cells. Moreover, the basal level of secreted TIMP-1 protein was very low in these cells as compared to the normal EVT cells. TGF-beta1 upregulated TIMP-1 mRNA and secreted protein in Smad3-restituted JAR cells as well as in the normal EVT cells, whereas no effect was detected in Smad3-deficient (wild-type) JAR cells. We had earlier shown that Smad3-restituted JAR cells had also restored TGF-beta response of plasminogen activator inhibitor-1 upregulation. However, in vitro functional analysis revealed that, in contrast to the normal EVT cells, anti-invasive action of TGF-beta was not restored in Smad3-restituted JAR cells. Thus, additional factors (possibly low expression of Smad4 and/or other unknown factors) may contribute to refractoriness to anti-invasive action of TGF-beta in JAR cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
300
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
383-90
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12504095-Cell Line, pubmed-meshheading:12504095-Choriocarcinoma, pubmed-meshheading:12504095-DNA-Binding Proteins, pubmed-meshheading:12504095-Female, pubmed-meshheading:12504095-Gene Expression Regulation, Neoplastic, pubmed-meshheading:12504095-Humans, pubmed-meshheading:12504095-Neoplasm Invasiveness, pubmed-meshheading:12504095-Pregnancy, pubmed-meshheading:12504095-RNA, Messenger, pubmed-meshheading:12504095-Smad3 Protein, pubmed-meshheading:12504095-Tissue Inhibitor of Metalloproteinase-1, pubmed-meshheading:12504095-Trans-Activators, pubmed-meshheading:12504095-Transfection, pubmed-meshheading:12504095-Transforming Growth Factor beta, pubmed-meshheading:12504095-Trophoblasts, pubmed-meshheading:12504095-Tumor Cells, Cultured, pubmed-meshheading:12504095-Up-Regulation, pubmed-meshheading:12504095-Uterine Neoplasms
pubmed:year
2003
pubmed:articleTitle
Reconstitution of Smad3 restores TGF-beta response of tissue inhibitor of metalloprotease-1 upregulation in human choriocarcinoma cells.
pubmed:affiliation
Department of Anatomy and Cell Biology, The University of Western Ontario, London, Ont. Canada N6A 5C1.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't