Linked Life Data

12491604

Source:http://linkedlifedata.com/resource/pubmed/id/12491604

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2002-12-20
pubmed:abstractText
An improvement trial for identification of the Mycobacterium tuberculosis complex by immunochromatographic assay using anti-MPB 64 monoclonal antibodies (MPB 64-ICA) is documented. The new MPB 64-ICA is very useful for simple and rapid identification of the Mycobacterium tuberculosis complex with specificity, but it is a little difficult to prove the Mycobacterium tuberculosis complex on the blood culture samples taken from the immunosuppressive or the AIDS patients, because the reactive bands are red or reddish-purple in both the control and positive zones of immunochromatographic assay, which are often indistinguishable from the background mixed with hemoglobin caused from the blood samples. We tried to remove the hemoglobin interference in the samples and was able to make clear the background with addition of hydrogen peroxide (H2O2). The results are followings; the optimum time and density of H2O2 are from 5 to 15 minutes and 35%, respectively. Accordingly, the improvement trial for identification of the Mycobacterium tuberculosis complex by immunochromatographic assay using MPB64-ICA with H2O2 is useful to identify the positive reactive band from the interference by hemoglobin.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0047-1860
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1067-71
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
[Improvement for identification of Mycobacterium tuberculosis complex by immunochromatographic assay anti-MPB 64 monoclonal antibodies with "H2O2" addition trial on blood culture samples].
pubmed:affiliation
Department of Clinical Laboratory, National Sanatorium Minami Yokohama Hospital, Yokohama 233-8503.
pubmed:publicationType
Journal Article, English Abstract