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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
2002-12-19
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pubmed:abstractText |
Transforming growth factor beta-1 (TGF beta-1) is a multifunctional growth factor that is expressed in numerous cell types. It has been shown to induce secretion of dentin extracellular matrix components associated with primary dentinogenesis and to play a role in tertiary or reparative dentinogenesis. In this study, we investigated the potential transcriptional regulation by TGF beta-1 of two dentin matrix proteins: dentin matrix protein 1 (DMP-1), and dentin sialophosphoprotein (DSPP). In vitro promoter studies were performed using plasmid constructs containing mouse DMP-1 and DSPP promoter sequences fused to the luciferase reporter gene. Constructs were transiently transfected in the mouse odontoblast cell line M06-G3 and cultured in the presence or absence of TGF beta-1. The integrity of the TGF beta-1 signaling pathway was investigated in the M06-G3 cells by identifying known key effectors of TGF beta-1 signal transduction. Transient transfection studies demonstrate for the first time that TGF beta-1 downregulates both DMP-1 and DSPP genes. Our findings indicate that the TGF beta-1 type I receptor ALK5 is expressed by odontoblasts as well as the signal transduction proteins Smad2, Smad3, and Smad4. These results suggest that TGF beta-1 regulates two key dentin proteins involved in matrix mineralization most likely mediated through the type I ALK5 receptor and transduced by Smads 2, 3, and 4.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Activin Receptors, Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Dmp1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transforming Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/TGF-beta type I receptor,
http://linkedlifedata.com/resource/pubmed/chemical/Tgfb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tgfb1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1,
http://linkedlifedata.com/resource/pubmed/chemical/dentin sialophosphoprotein
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pubmed:status |
MEDLINE
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pubmed:issn |
0300-8207
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
354-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12489180-Activin Receptors, Type I,
pubmed-meshheading:12489180-Animals,
pubmed-meshheading:12489180-Cell Line,
pubmed-meshheading:12489180-Down-Regulation,
pubmed-meshheading:12489180-Extracellular Matrix Proteins,
pubmed-meshheading:12489180-Immunohistochemistry,
pubmed-meshheading:12489180-Mice,
pubmed-meshheading:12489180-Odontoblasts,
pubmed-meshheading:12489180-Phosphoproteins,
pubmed-meshheading:12489180-Promoter Regions, Genetic,
pubmed-meshheading:12489180-Protein Precursors,
pubmed-meshheading:12489180-Protein-Serine-Threonine Kinases,
pubmed-meshheading:12489180-Receptors, Transforming Growth Factor beta,
pubmed-meshheading:12489180-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12489180-Sialoglycoproteins,
pubmed-meshheading:12489180-Transfection,
pubmed-meshheading:12489180-Transforming Growth Factor beta,
pubmed-meshheading:12489180-Transforming Growth Factor beta1
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pubmed:year |
2002
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pubmed:articleTitle |
TGF beta-1 downregulates DMP-1 and DSPP in odontoblasts.
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pubmed:affiliation |
Department of Pediatric Dentistry, Dental School, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, MCS 7888, San Antonio, Texas 78229-3900, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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