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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-11-4
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pubmed:abstractText |
Proteinase-activated receptor 2 (PAR2) is cleaved and activated by trypsin or mast cell tryptase, and may play an important role in inflammation. We have investigated the potential of PAR2 agonists to modulate TNF-alpha secretion from human astrocytoma cell line CCF-STTG1. We found that CCF-STTG1 expresses PAR2 by RT-PCR and Western blot analysis. Agonists such as trypsin, the peptide SLIGKV-NH(2) (corresponding to the PAR2 tethered ligand), or mast cell tryptase directly signal to CCF-STTG1 to stimulate secretion of TNF-alpha but do not stimulate in the presence of soybean trypsin inhibitor (SBTI) or VKGILS-NH(2) (reverse peptide). The secretion of TNF-alpha by trypsin was significantly blocked by pretreatment with either 50 microM PD98059 or 1 microM SB203580. Furthermore, trypsin stimulated the activation of extracellular signal-regulated kinase (ERK) and p38 MAP kinase homologue in CCF-STTG1 without any detectable activation of c-Jun N-terminal kinase (JNK). These results show that trypsin may induce TNF-alpha secretion following activation of ERK and p38 via PAR2 in CCF-STTG1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Chymases,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/PD 98059,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, PAR-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thrombin,
http://linkedlifedata.com/resource/pubmed/chemical/SB 203580,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0263-6484
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2002 John Wiley & Sons, Ltd.
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
339-45
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12415569-Astrocytoma,
pubmed-meshheading:12415569-Blotting, Western,
pubmed-meshheading:12415569-Calcium,
pubmed-meshheading:12415569-Central Nervous System,
pubmed-meshheading:12415569-Chymases,
pubmed-meshheading:12415569-Cytokines,
pubmed-meshheading:12415569-Enzyme Activation,
pubmed-meshheading:12415569-Enzyme Inhibitors,
pubmed-meshheading:12415569-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12415569-Flavonoids,
pubmed-meshheading:12415569-Humans,
pubmed-meshheading:12415569-Imidazoles,
pubmed-meshheading:12415569-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12415569-Peptides,
pubmed-meshheading:12415569-Polymerase Chain Reaction,
pubmed-meshheading:12415569-Protein Structure, Tertiary,
pubmed-meshheading:12415569-Pyridines,
pubmed-meshheading:12415569-RNA, Messenger,
pubmed-meshheading:12415569-Receptor, PAR-2,
pubmed-meshheading:12415569-Receptors, Thrombin,
pubmed-meshheading:12415569-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12415569-Serine Endopeptidases,
pubmed-meshheading:12415569-Time Factors,
pubmed-meshheading:12415569-Trypsin,
pubmed-meshheading:12415569-Trypsin Inhibitors,
pubmed-meshheading:12415569-Tumor Cells, Cultured,
pubmed-meshheading:12415569-Tumor Necrosis Factor-alpha,
pubmed-meshheading:12415569-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2002
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pubmed:articleTitle |
Agonists of proteinase-activated receptor 2 induce TNF-alpha secretion from astrocytoma cells.
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pubmed:affiliation |
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Cheonbuk 570-749, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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