Linked Life Data

12414794

Source:http://linkedlifedata.com/resource/pubmed/id/12414794

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
52
pubmed:dateCreated
2002-12-23
pubmed:abstractText
Epidermal growth factor induction of c-jun expression requires ATF1 and MEF2 sites in the c-jun promoter. We find that activation of the c-jun promoter through the ATF1 site requires phosphorylation of ATF1 at serine 63. A serine 63 to alanine mutation of ATF1 acts to block epidermal growth factor (EGF) induction of a transfected c-jun gene. ATF1 can be phosphorylated by mitogen- and stress-activated protein kinase 1 (MSK1), which is activated by EGF and ERK1/2. Kinase-dead MSK1 mutants blocked EGF induction of a transfected c-jun gene suggesting that MSK1 or a similar family member is required for induced c-jun expression. Use of the MEK1 inhibitor U0126 and dominant negative MEK1 further showed that MSK1 activation and c-jun induction require the ERK pathway. In contrast, a JNK inhibitor blocked EGF induction of c-jun expression but not ATF1 phosphorylation. These results show that the two MAPK pathways, ERK and JNK, are required for EGF-induced c-jun expression and that the ERK pathway acts through downstream phosphorylation of ATF1.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
50550-6
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12414794-Activating Transcription Factor 1, pubmed-meshheading:12414794-Animals, pubmed-meshheading:12414794-DNA-Binding Proteins, pubmed-meshheading:12414794-Enzyme Inhibitors, pubmed-meshheading:12414794-Epidermal Growth Factor, pubmed-meshheading:12414794-Gene Expression Regulation, pubmed-meshheading:12414794-Genes, jun, pubmed-meshheading:12414794-HeLa Cells, pubmed-meshheading:12414794-Humans, pubmed-meshheading:12414794-MAP Kinase Signaling System, pubmed-meshheading:12414794-Mice, pubmed-meshheading:12414794-Mitogen-Activated Protein Kinases, pubmed-meshheading:12414794-Phosphorylation, pubmed-meshheading:12414794-Promoter Regions, Genetic, pubmed-meshheading:12414794-Recombinant Proteins, pubmed-meshheading:12414794-Ribosomal Protein S6 Kinases, 90-kDa, pubmed-meshheading:12414794-Transcription Factors, pubmed-meshheading:12414794-Transfection
pubmed:year
2002
pubmed:articleTitle
ATF1 phosphorylation by the ERK MAPK pathway is required for epidermal growth factor-induced c-jun expression.
pubmed:affiliation
Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.