Linked Life Data

12412627

Source:http://linkedlifedata.com/resource/pubmed/id/12412627

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-11-4
pubmed:abstractText
Beta-defensins are cationic peptides produced by epithelial cells that have been proposed to be an important component of immune function at mucosal surfaces. Similarities between mammalian beta-defensins may permit the use of murine models to further define the role of these peptides in innate host defense. Murine beta-defensin-3 (mBD-3) is a peptide that exhibits homology at the gene level to human beta-defensin-2 (hBD-2), one of four beta-defensins identified in man. The purpose of this study was to determine the antimicrobial activity of mBD-3, the tissue distribution of mBD-3 expression, and the effect of gram-negative bacterial infection on mBD-3 expression. Based on the sequence deduced from mBD-3 cDNA, a 40-amino acid peptide was assembled using automated [n-(9-fluorenyl)methoxycarbonyl] solid-phase synthesis. The antimicrobial activity of synthetic mBD-3 was evaluated in microdilution broth assays using bacterial and fungal organisms. mBD-3 mRNA expression was assayed by polymerase chain reaction (PCR) using cDNA derived from a panel of tissues. Expression of mBD-3 was also evaluated in tissues obtained from mice 24 h after intraperitoneal infection with Escherichia coli using reverse transcriptase (RT)-PCR. Synthetic mBD-3 inhibited the growth of E. coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans at concentrations from 25 to 50 microg/mL. Constitutive expression of mBD-3 mRNA was not consistently found in any organ using RT-PCR. In an E. coli peritonitis model, expression of mBD-3 mRNA was upregulated only in the esophagus and tongue. We conclude that mBD-3 is an inducible peptide with limited tissue expression during E. coli peritonitis. Because it exhibits broad-spectrum antimicrobial activity, this peptide may serve as an innate defense against microbial invasion at specific mucosal surfaces in the mouse.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1073-2322
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
461-4
pubmed:dateRevised
2008-9-8
pubmed:meshHeading
pubmed-meshheading:12412627-Amino Acid Sequence, pubmed-meshheading:12412627-Animals, pubmed-meshheading:12412627-Anti-Bacterial Agents, pubmed-meshheading:12412627-Anti-Infective Agents, pubmed-meshheading:12412627-Candida albicans, pubmed-meshheading:12412627-Escherichia coli, pubmed-meshheading:12412627-Escherichia coli Infections, pubmed-meshheading:12412627-Female, pubmed-meshheading:12412627-Gene Expression, pubmed-meshheading:12412627-Humans, pubmed-meshheading:12412627-Male, pubmed-meshheading:12412627-Mice, pubmed-meshheading:12412627-Mice, Inbred BALB C, pubmed-meshheading:12412627-Molecular Sequence Data, pubmed-meshheading:12412627-Pseudomonas aeruginosa, pubmed-meshheading:12412627-Recombinant Proteins, pubmed-meshheading:12412627-Sepsis, pubmed-meshheading:12412627-Staphylococcus aureus, pubmed-meshheading:12412627-Tissue Distribution, pubmed-meshheading:12412627-beta-Defensins
pubmed:year
2002
pubmed:articleTitle
Murine beta-defensin-3 is an inducible peptide with limited tissue expression and broad-spectrum antimicrobial activity.
pubmed:affiliation
Robert Wood Johnson Medical School, Department of Surgery, New Brunswick, New Jersey 08903, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't