Linked Life Data

12408584

Source:http://linkedlifedata.com/resource/pubmed/id/12408584

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-5
pubmed:dateCreated
2002-10-31
pubmed:abstractText
Metallothioneins (MTs) are low molecular weight metal binding proteins that are implicated in heavy metal detoxification processes, and their quantification are commonly used as biomarkers of exposure to heavy metal ions. To verify the use of zebrafish MT gene promoter for aquatic toxicology studies, an 835 bp zebrafish MT (zMT) gene promoter with four putative metal regulatory elements (MREs) was isolated by DNA walking method and characterized for metal induction of gene transcription using transient expression assays in a zebrafish caudal fin cell line, SJD.1. The cloned promoter was linked upstream to a luciferase reporter gene and found to be responsive to administrations of Zn2+, Cd2+, and Cu2+ in SID.1 cell-line, but Hg2+, Ni2+, Pb2+, and Co2+ showed no significant induction of zMT gene transcription.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0141-1136
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
335-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
Characterization of zebrafish metallothionein gene promoter in a zebrafish caudal fin cell-line, SJD. 1.
pubmed:affiliation
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT.
pubmed:publicationType
Journal Article