Source:http://linkedlifedata.com/resource/pubmed/id/12406095
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2002-10-30
|
| pubmed:abstractText |
Umbilical cord blood (UCB) provides immediate access to haemopoietic stem/progenitor cells (HSPC) but low cell number restricts use in full adult bone marrow reconstitution. This study investigated early ex vivo expansion kinetics of UCB AC133+ cells (2-4 x 10(4)/ml), mononuclear cells (MNC, 1-2 x 10(6)/ml) and AC133negative cells (AC133(neg), 2-4 x 10(4)/ml) in stroma-free 8 d liquid culture (fetal bovine serum-supplemented Iscove's-modified Dulbecco's medium (IMDM) with either 'K36EG'[c-Kit ligand, interleukin 3 (IL-3), IL-6, erythropoietin, granulocyte colony-stimulating factor] or 'TPOFL' (thrombopoietin, Flt-3 ligand). Cell enumeration, apoptosis assay and AC133/CD34/CD38 antigen immunophenotyping were performed at d 0, 3, 5 and 8. All three cell populations went through a proliferation lag phase between d 3 and d 5. AC133+ cells recovered better from lag phase with significantly higher fold increase (FI) when compared with MNC and AC133(neg) populations (K36EG FI: 15.04 +/- 5.46; TPOFL FI: 8.59 +/- 2.92, P < 0.05). After 8 d, populations lacking AC133+ cells were significantly more inclined to undergo apoptosis under proliferative conditions (P < 0.01). Also, when compared with K36EG, 8 d TPOFL-expanded AC133+ cells encompassed a significantly higher percentage of AC133+ and CD34+ early HSPC (K36EG: 20.50 +/- 2.36; TPOFL: 47.00 +/- 7.69; P < 0.05). In conclusion, TPOFL synergism demonstrated the potential for AC133+ HSPC ex vivo expansion inducing self-renewal, early HSPC maintenance and promoting cell survival status.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AC133 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosyl Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/CD38 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombopoietin,
http://linkedlifedata.com/resource/pubmed/chemical/flt3 ligand protein
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0007-1048
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
119
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
516-24
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| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12406095-ADP-ribosyl Cyclase,
pubmed-meshheading:12406095-Antigens, CD,
pubmed-meshheading:12406095-Antigens, CD34,
pubmed-meshheading:12406095-Antigens, CD38,
pubmed-meshheading:12406095-Apoptosis,
pubmed-meshheading:12406095-Cell Division,
pubmed-meshheading:12406095-Cell Separation,
pubmed-meshheading:12406095-Cells, Cultured,
pubmed-meshheading:12406095-Fetal Blood,
pubmed-meshheading:12406095-Flow Cytometry,
pubmed-meshheading:12406095-Glycoproteins,
pubmed-meshheading:12406095-Hematopoietic Stem Cell Mobilization,
pubmed-meshheading:12406095-Hematopoietic Stem Cells,
pubmed-meshheading:12406095-Humans,
pubmed-meshheading:12406095-Membrane Glycoproteins,
pubmed-meshheading:12406095-Membrane Proteins,
pubmed-meshheading:12406095-Peptides,
pubmed-meshheading:12406095-Thrombopoietin
|
| pubmed:year |
2002
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| pubmed:articleTitle |
AC133+ umbilical cord blood progenitors demonstrate rapid self-renewal and low apoptosis.
|
| pubmed:affiliation |
King-George Laboratory, St George's Hospital Medical School and Kingston University, London, UK.
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| pubmed:publicationType |
Journal Article,
Comparative Study
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