Source:http://linkedlifedata.com/resource/pubmed/id/12388444
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2003-1-16
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| pubmed:abstractText |
The purpose of this study was to determine the role of cyclooxygenase-2 (COX-2) and its metabolites in lower urinary tract function after induction of acute (4 h), intermediate (48 h), or chronic (10 day) cyclophosphamide (CYP)-induced cystitis. Bladders were harvested from euthanized female rats for analyses. Conscious cystometry was used to assess the effects of a COX-2-specific inhibitor, 5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl2(5H)-furanone (DFU, 5 mg/kg sc), a disubstituted furanone, in CYP-induced cystitis. COX-2 mRNA was increased in inflamed bladders after acute (12-fold) and chronic (9-fold) treatment. COX-2 protein expression in inflamed bladders paralleled COX-2 mRNA expression. Prostaglandin D2-methoxime expression in the bladder was significantly (P < or = 0.01) increased in acute (3-fold) and chronic (5.5-fold) cystitis. Prostaglandin E2 was significantly (P < or = 0.01) increased (2-fold) in the bladder with intermediate (1.7-fold) and chronic (2.6-fold) cystitis. COX-2-immunoreactive cell profiles were distributed throughout the inflamed bladder and coexpressed histamine immunoreactivity. Conscious cystometry in rats treated with CYP + DFU showed increased micturition intervals 4 and 48 h after CYP treatment and decreased intravesical pressures during filling and micturition compared with rats treated with CYP + vehicle. These studies suggest an involvement of urinary bladder COX-2 and its metabolites in altered micturition reflexes with CYP-induced cystitis.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/11-methoxime prostaglandin D2,
http://linkedlifedata.com/resource/pubmed/chemical/5,5-dimethyl-3-(3-fluorophenyl)-4-(4...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclophosphamide,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Furans,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin D2,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandins, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0363-6119
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
284
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
R574-85
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:12388444-Animals,
pubmed-meshheading:12388444-Blotting, Western,
pubmed-meshheading:12388444-Cyclooxygenase 2,
pubmed-meshheading:12388444-Cyclophosphamide,
pubmed-meshheading:12388444-Cystitis,
pubmed-meshheading:12388444-Dinoprostone,
pubmed-meshheading:12388444-Drug Administration Schedule,
pubmed-meshheading:12388444-Female,
pubmed-meshheading:12388444-Furans,
pubmed-meshheading:12388444-Gene Expression Regulation,
pubmed-meshheading:12388444-Immunoenzyme Techniques,
pubmed-meshheading:12388444-Immunohistochemistry,
pubmed-meshheading:12388444-Isoenzymes,
pubmed-meshheading:12388444-Prostaglandin D2,
pubmed-meshheading:12388444-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:12388444-Prostaglandins, Synthetic,
pubmed-meshheading:12388444-RNA, Messenger,
pubmed-meshheading:12388444-Rats,
pubmed-meshheading:12388444-Rats, Wistar,
pubmed-meshheading:12388444-Urinary Bladder,
pubmed-meshheading:12388444-Urination
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| pubmed:year |
2003
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| pubmed:articleTitle |
COX-2 and prostanoid expression in micturition pathways after cyclophosphamide-induced cystitis in the rat.
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| pubmed:affiliation |
Departments of Neurology, Anatomy and Neurobiology, and Surgery, University of Vermont College of Medicine, Burlington, Vermont 05405, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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