Source:http://linkedlifedata.com/resource/pubmed/id/12354693
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
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| pubmed:dateCreated |
2002-10-31
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| pubmed:abstractText |
Induction of tumor cell migration is a key step in invasion and metastasis. Here we report that the epidermal growth factor (EGF)-induced cell migration of breast cancer cells is attributed to a transient, rather than a sustained, activation of phospholipase C (PLC)-gamma1 due to c-erbB-2 signaling. EGF stimulation of EGF receptor (EGFR) overexpressing cells resulted in long-term PLC-gamma1 tyrosine phosphorylation and sustained levels of inositol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG) producing sinusoidal calcium oscillations. In contrast, c-erbB-2/EGFR expressing cells displayed baseline transient calcium oscillations after EGF treatment due to short-term PLC-gamma1 tyrosine phosphorylation and short-term IP3 and DAG turnover. A third cell line expressing a point-mutated c-erbB-2 receptor that lacks the autophosphorylation Y1248 was generated to investigate whether the different PLC-gamma1 activation was attributed to this structure. Neither PLC-gamma1 tyrosine phosphorylation nor IP3 and DAG turnover and calcium oscillations were observed in this cell line, indicating the modulation of the PLC-g1 activation time course by c-erbB-2 signaling. Induction of cell migration was solely observable in the c-erbB-2-positive cell line as proved by the mode of actin reorganization and a cell migration assay, using a 3D-collagen lattice. In summary, c-erbB-2 up-regulation switches on the cell migration program by modulating the time course of PLC-gamma1 activation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(6-((3-methoxyestra-1,3,5(10)-trie...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Estrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase C gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidinones,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-2,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
1530-6860
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| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:volume |
16
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1823-5
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:12354693-Calcium,
pubmed-meshheading:12354693-Cell Movement,
pubmed-meshheading:12354693-Dimerization,
pubmed-meshheading:12354693-Enzyme Activation,
pubmed-meshheading:12354693-Epidermal Growth Factor,
pubmed-meshheading:12354693-Estrenes,
pubmed-meshheading:12354693-Humans,
pubmed-meshheading:12354693-Isoenzymes,
pubmed-meshheading:12354693-Phosphodiesterase Inhibitors,
pubmed-meshheading:12354693-Phospholipase C gamma,
pubmed-meshheading:12354693-Phosphorylation,
pubmed-meshheading:12354693-Pyrrolidinones,
pubmed-meshheading:12354693-Receptor, Epidermal Growth Factor,
pubmed-meshheading:12354693-Receptor, erbB-2,
pubmed-meshheading:12354693-Signal Transduction,
pubmed-meshheading:12354693-Tumor Cells, Cultured,
pubmed-meshheading:12354693-Type C Phospholipases,
pubmed-meshheading:12354693-Tyrosine
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| pubmed:year |
2002
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| pubmed:articleTitle |
Induction of cancer cell migration by epidermal growth factor is initiated by specific phosphorylation of tyrosine 1248 of c-erbB-2 receptor via EGFR.
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| pubmed:affiliation |
Institute of Immunology, University of Witten/Herdecke, Stockumer Str. 10, 58448 Witten, Germany. thomasd@uni-wh.de
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| pubmed:publicationType |
Journal Article
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