Linked Life Data

12233782

Source:http://linkedlifedata.com/resource/pubmed/id/12233782

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:12233782rdf:typepubmed:Citationlld:pubmed
pubmed-article:12233782lifeskim:mentionsumls-concept:C0010453lld:lifeskim
pubmed-article:12233782lifeskim:mentionsumls-concept:C0034493lld:lifeskim
pubmed-article:12233782lifeskim:mentionsumls-concept:C0021753lld:lifeskim
pubmed-article:12233782lifeskim:mentionsumls-concept:C0083867lld:lifeskim
pubmed-article:12233782lifeskim:mentionsumls-concept:C0225369lld:lifeskim
pubmed-article:12233782lifeskim:mentionsumls-concept:C0521339lld:lifeskim
pubmed-article:12233782lifeskim:mentionsumls-concept:C0017262lld:lifeskim
pubmed-article:12233782lifeskim:mentionsumls-concept:C0205263lld:lifeskim
pubmed-article:12233782lifeskim:mentionsumls-concept:C2911684lld:lifeskim
pubmed-article:12233782lifeskim:mentionsumls-concept:C0185117lld:lifeskim
pubmed-article:12233782pubmed:issue2lld:pubmed
pubmed-article:12233782pubmed:dateCreated2002-9-17lld:pubmed
pubmed-article:12233782pubmed:abstractTextTo elucidate the effects of interleukin-1beta (IL-1beta) on osteogenic protein-1 (OP-1) gene expression in a polylayer culture of rabbit articular chondrocytes, we measured rabbit OP-1 mRNA using quantitative TaqMan reverse transcriptase-polymerase chain reaction (RT-PCR) techniques. Rabbit articular chondrocytes were isolated and cultured in minimum essential medium eagle alpha modification containing 10% fetal bovine serum for 7 days. IL-1beta was then added and cultures were continued for 48 or 96 hours. OP-1 gene expression was detected in cell cultures both with and without addition of IL-1beta. However, the level of expression was very low in the control group. OP-1 gene expression was significantly increased about 450- to 800-fold in IL-1beta-treated groups (0.1, 1, and 10 ng/ml) versus the control group. Evaluation of serial changes in OP-1 expression after addition of IL-1beta (10 ng/ml) revealed that OP-1 gene expression increased rapidly after addition of IL-1beta, reaching a peak at 48 hours, and then decreasing. Simultaneous assay of CD44 expression demonstrated a rapid increase, similar to that of OP-1 expression, following addition of IL-1beta: this was followed by a more gradual increase. Assay of hyaluronan synthase-2 (HAS-2) expression following addition of IL-1beta showed an increase after OP-1 expression had already reached a peak. Our results demonstrate that OP-1 expression is induced by IL-1beta and suggest that this expression, like that of HAS-2, may play a role as a protective mechanism against inflammatory cytokines.lld:pubmed
pubmed-article:12233782pubmed:languageenglld:pubmed
pubmed-article:12233782pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12233782pubmed:citationSubsetIMlld:pubmed
pubmed-article:12233782pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12233782pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12233782pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12233782pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12233782pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12233782pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12233782pubmed:statusMEDLINElld:pubmed
pubmed-article:12233782pubmed:monthJunlld:pubmed
pubmed-article:12233782pubmed:issn0040-8727lld:pubmed
pubmed-article:12233782pubmed:authorpubmed-author:ShimamuraTada...lld:pubmed
pubmed-article:12233782pubmed:authorpubmed-author:YoshidaSatosh...lld:pubmed
pubmed-article:12233782pubmed:authorpubmed-author:KubotaYoshiko...lld:pubmed
pubmed-article:12233782pubmed:authorpubmed-author:TobaTamotsuTlld:pubmed
pubmed-article:12233782pubmed:authorpubmed-author:HoriuchiSabur...lld:pubmed
pubmed-article:12233782pubmed:issnTypePrintlld:pubmed
pubmed-article:12233782pubmed:volume197lld:pubmed
pubmed-article:12233782pubmed:ownerNLMlld:pubmed
pubmed-article:12233782pubmed:authorsCompleteYlld:pubmed
pubmed-article:12233782pubmed:pagination101-9lld:pubmed
pubmed-article:12233782pubmed:dateRevised2008-11-21lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:meshHeadingpubmed-meshheading:12233782...lld:pubmed
pubmed-article:12233782pubmed:year2002lld:pubmed
pubmed-article:12233782pubmed:articleTitleInduction of osteogenic protein-1 expression by interleukin-1beta in cultured rabbit articular chondrocytes.lld:pubmed
pubmed-article:12233782pubmed:affiliationDepartment of Biochemistry, Iwate Medical University, Morioka, Japan. satto@fj8.so-net.ne.jplld:pubmed
pubmed-article:12233782pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12233782pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12233782lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12233782lld:pubmed