Source:http://linkedlifedata.com/resource/pubmed/id/12225995
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2002-9-12
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| pubmed:abstractText |
Alternative splicing of the human glucocorticoid receptor (GR) primary transcript generates two protein isoforms: GR-alpha and GR-beta. We investigated the expression of both GR isoforms in healthy human cells and tissues. GR-alpha mRNA abundance (x10(6) cDNA copies/microg total RNA) was as follows: brain (3.83 +/- 0.80) > skeletal muscle > macrophages > lung > kidney > liver > heart > eosinophils > peripheral blood mononuclear cells (PBMCs) > nasal mucosa > neutrophils > colon (0.33 +/- 0.04). GR-beta mRNA was much less expressed than GR-alpha mRNA. Its abundance (x10(3) cDNA copies/microg total RNA) was as follows: eosinophils (1.55 +/- 0.58) > PBMCs > liver > or = skeletal muscle > kidney > macrophages > lung > neutrophils > brain > or = nasal mucosa > heart (0.15 +/- 0.08). GR-beta mRNA was not found in colon. While GR-alpha protein was detected in all cells and tissues, GR-beta was not detected in any specimen. Our results suggest that, in physiological conditions, the default splicing pathway is the one leading to GR-alpha. The alternative splicing event leading to GR-beta is minimally activated.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glucocorticoid,
http://linkedlifedata.com/resource/pubmed/chemical/glucocorticoid receptor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/glucocorticoid receptor beta
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0363-6143
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
283
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
C1324-31
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| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12225995-Animals,
pubmed-meshheading:12225995-Brain Chemistry,
pubmed-meshheading:12225995-COS Cells,
pubmed-meshheading:12225995-Cell Line,
pubmed-meshheading:12225995-Colon,
pubmed-meshheading:12225995-Humans,
pubmed-meshheading:12225995-Kidney,
pubmed-meshheading:12225995-Leukocytes,
pubmed-meshheading:12225995-Liver,
pubmed-meshheading:12225995-Lung,
pubmed-meshheading:12225995-Muscle, Skeletal,
pubmed-meshheading:12225995-Myocardium,
pubmed-meshheading:12225995-Nasal Mucosa,
pubmed-meshheading:12225995-Organ Specificity,
pubmed-meshheading:12225995-Phagocytes,
pubmed-meshheading:12225995-Protein Isoforms,
pubmed-meshheading:12225995-RNA, Messenger,
pubmed-meshheading:12225995-Receptors, Glucocorticoid,
pubmed-meshheading:12225995-Reference Values
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| pubmed:year |
2002
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| pubmed:articleTitle |
Expression of glucocorticoid receptor alpha- and beta-isoforms in human cells and tissues.
|
| pubmed:affiliation |
Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic, Departament de Medicina, Universitat de Barcelona, 08036 Barcelona, Spain.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|