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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2002-9-9
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pubmed:abstractText |
Fas ligand and TNF-related apoptosis-inducing ligand (TRAIL) induce apoptosis in many different cell types. Jurkat T cells die rapidly by apoptosis after treatment with either ligand. We have previously shown that mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) can act as a negative regulator of apoptosis mediated by the Fas receptor. In this study we examined whether MAPK/ERK can also act as a negative regulator of apoptosis induced by TRAIL. Activated Jurkat T cells were efficiently protected from TRAIL-induced apoptosis. The protection was shown to be MAPK/ERK dependent and independent of protein synthesis. MAPK/ERK suppressed TRAIL-induced apoptosis upstream of the mitochondrial amplification loop because mitochondrial depolarization and release of cytochrome c were inhibited. Furthermore, caspase-8-mediated relocalization and activation of Bid, a proapoptotic member of the Bcl family, was also inhibited by the MAPK/ERK signaling. The protection occurred at the level of the apoptotic initiator caspase-8, as the cleavage of caspase-8 was inhibited but the assembly of the death-inducing signaling complex was unaffected. Both TRAIL and Fas ligand have been suggested to regulate the clonal size and persistence of different T cell populations. Our previous results indicate that MAPK/ERK protects recently activated T cells from Fas receptor-mediated apoptosis during the initial phase of an immune response before the activation-induced cell death takes place. The results of this study show clearly that MAPK/ERK also participates in the inhibition of TRAIL-induced apoptosis after T cell activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CASP8 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CASP9 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 8,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 9,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome c Group,
http://linkedlifedata.com/resource/pubmed/chemical/FADD protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fas-Associated Death Domain Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/MAP2K1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Muromonab-CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, TNF-Related...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/TNF-Related Apoptosis-Inducing...,
http://linkedlifedata.com/resource/pubmed/chemical/TNFRSF10A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TNFRSF10B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TNFSF10 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2851-60
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12218097-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:12218097-Antigens, CD95,
pubmed-meshheading:12218097-Apoptosis,
pubmed-meshheading:12218097-Apoptosis Regulatory Proteins,
pubmed-meshheading:12218097-Biological Transport, Active,
pubmed-meshheading:12218097-Carrier Proteins,
pubmed-meshheading:12218097-Caspase 8,
pubmed-meshheading:12218097-Caspase 9,
pubmed-meshheading:12218097-Caspases,
pubmed-meshheading:12218097-Cell Membrane,
pubmed-meshheading:12218097-Cytochrome c Group,
pubmed-meshheading:12218097-Enzyme Activation,
pubmed-meshheading:12218097-Fas-Associated Death Domain Protein,
pubmed-meshheading:12218097-Humans,
pubmed-meshheading:12218097-Immunosuppressive Agents,
pubmed-meshheading:12218097-Intracellular Membranes,
pubmed-meshheading:12218097-Jurkat Cells,
pubmed-meshheading:12218097-Lymphocyte Activation,
pubmed-meshheading:12218097-MAP Kinase Kinase 1,
pubmed-meshheading:12218097-MAP Kinase Signaling System,
pubmed-meshheading:12218097-Membrane Glycoproteins,
pubmed-meshheading:12218097-Membrane Potentials,
pubmed-meshheading:12218097-Mitochondria,
pubmed-meshheading:12218097-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:12218097-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12218097-Muromonab-CD3,
pubmed-meshheading:12218097-Protein-Serine-Threonine Kinases,
pubmed-meshheading:12218097-Receptors, TNF-Related Apoptosis-Inducing Ligand,
pubmed-meshheading:12218097-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:12218097-T-Lymphocytes,
pubmed-meshheading:12218097-TNF-Related Apoptosis-Inducing Ligand,
pubmed-meshheading:12218097-Tetradecanoylphorbol Acetate,
pubmed-meshheading:12218097-Tumor Necrosis Factor-alpha
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pubmed:year |
2002
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pubmed:articleTitle |
Mitogen-activated protein kinase/extracellular signal-regulated kinase signaling in activated T cells abrogates TRAIL-induced apoptosis upstream of the mitochondrial amplification loop and caspase-8.
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pubmed:affiliation |
Turku Center for Biotechnology, University of Turku and Abo Akademi University, BioCity, Finland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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