|
rdf:type |
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lifeskim:mentions |
umls-concept:C0010531,
umls-concept:C0033634,
umls-concept:C0056695,
umls-concept:C0109317,
umls-concept:C0178719,
umls-concept:C0282549,
umls-concept:C0563533,
umls-concept:C0752312,
umls-concept:C0851827,
umls-concept:C1120843,
umls-concept:C1148673,
umls-concept:C1150579,
umls-concept:C1333340,
umls-concept:C1366882,
umls-concept:C1370600,
umls-concept:C1701901,
umls-concept:C1705767,
umls-concept:C1705791,
umls-concept:C1879547
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pubmed:issue |
4
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pubmed:dateCreated |
2002-8-29
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pubmed:abstractText |
To investigate the possible mechanism of gene transcription changes induced by magnetic field (MF), we examined the DNA binding behavior of the transcription factor cyclic-AMP responsive element binding protein (CREB) in HL60 cells after exposure to a 0.1mT 50-Hz extremely low frequency (ELF) sinusoidal MF by a gel shift assay. Magnetic field induced a time-dependent activation of CREB binding. The complex formation increased shortly after MF exposure for 10min, reaching a peak level after 1h, and then recovered to basal level at 4h after exposure. A novel MF-induced ATF2/ATF2 homodimer formation was observed after MF exposure for 30min, 1, and 2h. Furthermore, We found that the MF-induced increase of CREB DNA binding in HL60 cells was dependent on both extracellular and intracellular Ca(2+) but not PKA, PKC, ERK, or p38 MAPK by using various pathway inhibitors. These data indicate that MF exposure activates CREB DNA binding through calcium-related signal transduction pathways under our experimental conditions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
296
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1013-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12200150-Calcium,
pubmed-meshheading:12200150-Cell Nucleus,
pubmed-meshheading:12200150-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:12200150-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:12200150-DNA,
pubmed-meshheading:12200150-Electromagnetic Fields,
pubmed-meshheading:12200150-HL-60 Cells,
pubmed-meshheading:12200150-Humans,
pubmed-meshheading:12200150-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12200150-Protein Binding,
pubmed-meshheading:12200150-Protein Kinase C,
pubmed-meshheading:12200150-Signal Transduction,
pubmed-meshheading:12200150-Time Factors,
pubmed-meshheading:12200150-Transcription, Genetic,
pubmed-meshheading:12200150-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2002
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pubmed:articleTitle |
CREB DNA binding activation by a 50-Hz magnetic field in HL60 cells is dependent on extra- and intracellular Ca(2+) but not PKA, PKC, ERK, or p38 MAPK.
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pubmed:affiliation |
Laboratory of Molecular and Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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