|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017638,
umls-concept:C0020964,
umls-concept:C0024432,
umls-concept:C0027651,
umls-concept:C0027950,
umls-concept:C0033414,
umls-concept:C0079784,
umls-concept:C0205253,
umls-concept:C0205263,
umls-concept:C0205373,
umls-concept:C0524466,
umls-concept:C0596901,
umls-concept:C1261381,
umls-concept:C1704259,
umls-concept:C1705987
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2002-7-31
|
|
pubmed:abstractText |
Cloned T9-C2 glioma cells transfected with membrane macrophage colony-stimulating factor (mM-CSF) never formed subcutaneous tumors when implanted into Fischer rats, whereas control T9 cells did. The T9-C2 cells were completely killed within 1 day through a mechanism that resembled paraptosis. Vacuolization of the T9-C2 cell's mitochondria and endoplasmic reticulum started within 4 hours after implantation. By 24 hours, the dead tumor cells were swollen and terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL)-positive. Bcl2-transduced T9-C2 cells failed to form tumors in rats. Both T9 and T9-C2 cells produced cytokine-induced neutrophil chemoattractant that recruited the granulocytes into the tumor injection sites, where they interacted with the tumor cells. Freshly isolated macrophages killed the T9-C2 cells in vitro by a mechanism independent of phagocytosis. Nude athymic rats treated with antiasialo GM1 antibody formed T9-C2 tumors, whereas rats treated with a natural killer cell (NK)-specific antibody failed to form tumors. When treated with antipolymorphonuclear leukocyte (anti-PMN) and antimacrophage antibodies, 80% of nude rats formed tumors, whereas only 40% of the rats developed a tumor when a single antibody was used. This suggests that both PMNs and macrophages are involved in the killing of T9-C2 tumor cells. Immunocompetent rats that rejected the living T9-C2 cells were immune to the intracranial rechallenge with T9 cells. No vaccinating effect occurred if the T9-C2 cells were freeze-thawed, x-irradiated, or treated with mitomycin-C prior to injection. Optimal tumor immunization using mM-CSF-transduced T9 cells requires viable tumor cells. In this study optimal tumor immunization occurred when a strong inflammatory response at the injection of the tumor cells was induced.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0006-4971
|
|
pubmed:author |
pubmed-author:ArpajirakulNearyN,
pubmed-author:BoussaidOO,
pubmed-author:ChenYijunY,
pubmed-author:DanQinghongQ,
pubmed-author:DelgadoChristinaC,
pubmed-author:DouglassThomasT,
pubmed-author:JadusMartin RMR,
pubmed-author:JeffesEdward W BEW,
pubmed-author:KimRonald CRC,
pubmed-author:KleinmanMichaelM,
pubmed-author:SanchezRamonR,
pubmed-author:WilliamsChristopher CCC,
pubmed-author:XuQingchengQ
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
100
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1373-80
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:12149220-Animals,
pubmed-meshheading:12149220-Antibodies,
pubmed-meshheading:12149220-Brain Neoplasms,
pubmed-meshheading:12149220-Cell Death,
pubmed-meshheading:12149220-Chemokines, CXC,
pubmed-meshheading:12149220-Chemotactic Factors,
pubmed-meshheading:12149220-Female,
pubmed-meshheading:12149220-Freezing,
pubmed-meshheading:12149220-Gene Expression,
pubmed-meshheading:12149220-Glioma,
pubmed-meshheading:12149220-Growth Substances,
pubmed-meshheading:12149220-Hot Temperature,
pubmed-meshheading:12149220-Immunization,
pubmed-meshheading:12149220-In Situ Nick-End Labeling,
pubmed-meshheading:12149220-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:12149220-Killer Cells, Natural,
pubmed-meshheading:12149220-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:12149220-Macrophages,
pubmed-meshheading:12149220-Microscopy, Electron,
pubmed-meshheading:12149220-Mitomycin,
pubmed-meshheading:12149220-Neutrophils,
pubmed-meshheading:12149220-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:12149220-Rats,
pubmed-meshheading:12149220-Rats, Inbred F344,
pubmed-meshheading:12149220-Transfection,
pubmed-meshheading:12149220-Tumor Cells, Cultured,
pubmed-meshheading:12149220-X-Rays
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Living T9 glioma cells expressing membrane macrophage colony-stimulating factor produce immediate tumor destruction by polymorphonuclear leukocytes and macrophages via a "paraptosis"-induced pathway that promotes systemic immunity against intracranial T9 gliomas.
|
|
pubmed:affiliation |
Diagnostic and Molecular Health Care Group, Veterans Affairs Medical Center, Long Beach, CA 90822, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
