Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2002-7-26
pubmed:abstractText
The promyelocytic leukaemia (PML) gene, which encodes a transformation and growth suppressor, was found to regulate transcription and apoptosis. PML was first identified at the chromosomal translocation break-points t(15;17) of acute promyelocytic leukaemia and the gene product may mediate cell-cycle control and apoptosis. PML was found to interact with the co-transactivator CREB binding protein (CBP) and the apoptotic-modulator Bax. To determine if PML, CBP and Bax may be involved in solid tumours, such as the nasopharyngeal carcinoma (NPC), a rare neoplasia that is prevalent in Southern China, the expression of these proteins and the proliferation marker Ki-67 was analysed by immunohistochemical staining. Expression of PML in the PML-oncogenic domain (POD) or nuclear bodies in most NPC was inversely correlated with the expression of Ki-67. In addition, based on PML expression patterns in NPC three subtypes could be identified, namely, Subtype-1, with strong PML expression in POD structures and with low Ki-67 staining; Subtype-2, where PML was expressed in a homogeneously diffused pattern, but with a low intensity in the tumour cells; while Ki-67 was expressed in a moderate number of cells and Subtype-3, where the majority of tumour cells were PML-negative, while a considerable number of tumour cells were strongly labelled with Ki-67. Furthermore, CBP was present in most of the NPC cells with moderate-strong nuclear staining, while the expression in non-tumour cells were relatively weak. However, there was no direct correlation between PML and CBP expression in the NPC examined. In addition, there was low or no expression of Bax in the NP and NPC. This is, to our knowledge, the first report describing PML and CBP expression in NPC and our data strongly suggests that PML and CBP, but not Bax, may play a role in the transformed phenotypes of NPC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/BAX protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CREB-Binding Protein, http://linkedlifedata.com/resource/pubmed/chemical/CREBBP protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Ki-67 Antigen, http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/PML protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0959-8049
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1600-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12142048-CREB-Binding Protein, pubmed-meshheading:12142048-Female, pubmed-meshheading:12142048-Gene Expression, pubmed-meshheading:12142048-Genes, Tumor Suppressor, pubmed-meshheading:12142048-Humans, pubmed-meshheading:12142048-Immunohistochemistry, pubmed-meshheading:12142048-Ki-67 Antigen, pubmed-meshheading:12142048-Male, pubmed-meshheading:12142048-Nasopharyngeal Neoplasms, pubmed-meshheading:12142048-Neoplasm Proteins, pubmed-meshheading:12142048-Nuclear Proteins, pubmed-meshheading:12142048-Proto-Oncogene Proteins, pubmed-meshheading:12142048-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:12142048-Trans-Activators, pubmed-meshheading:12142048-Transcription Factors, pubmed-meshheading:12142048-Tumor Suppressor Proteins, pubmed-meshheading:12142048-bcl-2-Associated X Protein
pubmed:year
2002
pubmed:articleTitle
Differential expression of the suppressor PML and Ki-67 identifies three subtypes of human nasopharyngeal carcinoma.
pubmed:affiliation
Institute of Radiological Sciences, National Yang Ming University, 155 Sec.2, Li-Nung St., Shi-Pai, ROC, Taipei, Taiwan. jchan@rics.bwh.harvard.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't