Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2002-7-15
pubmed:abstractText
Infections with Neisseria meningitidis are characterized by life-threatening meningitis and septicemia. The meningococcal porin proteins from serogroup B meningococci have been identified as candidates for inclusion in vaccines to prevent such infections. In this study, we investigated the vaccine potential of the PorB porin protein free of other meningococcal components. The porB gene from a strain of Neisseria meningitidis expressing the class 3 outer membrane porin protein (PorB3) was cloned into the pRSETB vector, and the protein was expressed at high levels in a heterologous host Escherichia coli. The recombinant protein was purified to homogeneity by affinity chromatography and used for immunization after incorporation into liposomes and into micelles composed either of zwitterionic detergent or nondetergent sulfobetaine. The immunogenicity of these preparations was compared to recombinant PorB protein adsorbed to Al(OH)(3) adjuvant as a control. Although sera raised against the protein adsorbed to Al(OH)(3) reacted with the purified recombinant protein, sera raised against liposomes and micelles showed greater activity with native protein, as measured by enzyme immunoassay with outer membranes and by whole-cell immunofluorescence. Reactivity with native protein was considerably enhanced by incorporation of the adjuvant monophosphoryl lipid A into the liposome or micelle preparations. Recognition of the native protein was in a serotype-specific manner and was associated with the ability of the antisera to promote high levels of serotype-specific complement-mediated killing of meningococci. These results demonstrate that the PorB protein should be considered as a component of a vaccine designed to prevent serogroup B meningococcal infection.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-10225920, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-10501243, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-10720549, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-11163678, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-11257368, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-11349046, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-11421893, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-1323620, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-1357461, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-1652557, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-1682541, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-1705642, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-1706758, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-2503673, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-2510417, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-2878967, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-3141555, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-4977281, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-7483804, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-7503420, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-7591118, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-7681519, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-7691983, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-7826351, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-7927704, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-8132350, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-8156052, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-8406872, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-8578833, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-8699067, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-8843630, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-9286045, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-9498448, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-9562686, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-9806046, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-9846738, http://linkedlifedata.com/resource/pubmed/commentcorrection/12117908-9916067
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4028-34
pubmed:dateRevised
2011-4-7
pubmed:meshHeading
pubmed-meshheading:12117908-Animals, pubmed-meshheading:12117908-Antibodies, Bacterial, pubmed-meshheading:12117908-Cloning, Molecular, pubmed-meshheading:12117908-Disease Models, Animal, pubmed-meshheading:12117908-Escherichia coli, pubmed-meshheading:12117908-Fluorescent Antibody Technique, pubmed-meshheading:12117908-Gene Expression, pubmed-meshheading:12117908-Genes, Bacterial, pubmed-meshheading:12117908-Immunoglobulin Isotypes, pubmed-meshheading:12117908-Meningococcal Infections, pubmed-meshheading:12117908-Meningococcal Vaccines, pubmed-meshheading:12117908-Mice, pubmed-meshheading:12117908-Mice, Inbred BALB C, pubmed-meshheading:12117908-Neisseria meningitidis, pubmed-meshheading:12117908-Porins, pubmed-meshheading:12117908-Recombinant Fusion Proteins, pubmed-meshheading:12117908-Vaccination, pubmed-meshheading:12117908-Vaccines, Synthetic
pubmed:year
2002
pubmed:articleTitle
Immunization with the recombinant PorB outer membrane protein induces a bactericidal immune response against Neisseria meningitidis.
pubmed:affiliation
Molecular Microbiology and Infection, Division of Infection, Inflammation and Repair, University of Southampton Medical School, and Southampton General Hospital, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't