Linked Life Data

12111977

Source:http://linkedlifedata.com/resource/pubmed/id/12111977

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-7-11
pubmed:abstractText
This study reports on the successful fabrication of artificial nerves with tissue engineering methods. Schwann cells were cultured for 2 weeks, seeded on polyglactin 910 scaffolds, and biomembrane-coated with rat-tail glue and laminin. Observation of the scaffolds' adsorptivity to Schwann cells, and of the growth and migration of Schwann cells, was made using a light microscope, and by scanning and transmission electron microscopy. The Schwann cells were able to migrate and proliferate on the polyglactin 910 fiber. Schwann cells were well-distributed, and formed a Büngner band on which the Schwann cells produced more matrices. Schwann cells on the biomembrane also grew well. We investigated the role of the tissue engineering conduit guide in vivo, using an established rabbit peripheral nerve regeneration model. At 8 weeks, axonal regeneration was observed in the distal nerve stump. Adult Schwann cells can be produced on the coated fiber and the biomembrane. Three-dimensional scaffolds with Schwann cells had the basic characteristics of the artificial nerve. These findings will provide a practical method for fabricating tissue-engineered artificial nerve to repair long nerve defects.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0738-1085
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-7
pubmed:dateRevised
2006-4-17
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Fabricating autologous tissue to engineer artificial nerve.
pubmed:affiliation
Department of Orthopedic Surgery, Zhon Shan Hospital, Fudan University Medical Center, 136 Yi Xue Yuan Road, Shanghai 200032, PR China. cbiaob@sohu.com
pubmed:publicationType
Journal Article