Linked Life Data

12074844

Source:http://linkedlifedata.com/resource/pubmed/id/12074844

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-6-20
pubmed:abstractText
Optimal experimental conditions for the delivery of phosphodiester or phosphorothioate antisense oligonucleotides (P-ASO/S-ASO) to PC12 cells were determined. Fluorescently labeled P-ASO or S-ASO were transfected to PC12 cells and the uptake of antisense, free or entrapped in liposomes, was monitored by confocal and fluorescent microscopy. Efficient delivery of fluorescently labeled ASO with low rates of cell death was obtained when PC12 cells were transfected with liposomes in Opti-MEM medium supplemented with sera, compared with control experiments where nonliposomal ASO were transfected to PC12 cells in sera-free media. Compared with P-ASO, the application of S-ASO for antisense studies in PC12 cells is more suitable due to the lower concentration required for an efficient antisense uptake and its higher intracellular stability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0168-0102
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Delivery of antisense oligonucleotides to PC12 cells.
pubmed:affiliation
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN, Mexico.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't