Linked Life Data

12051774

Source:http://linkedlifedata.com/resource/pubmed/id/12051774

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pubmed-article:12051774pubmed:abstractTextHIV-1(LAV-1) particles were collected by ultracentrifugation, treated with subtilisin, and then purified by Sepharose CL-4B column chromatography to remove microvesicles. The lysate of the purified human immunodeficiency virus type 1 (HIV-1) particles was subjected to two-dimensional (2D) gel electrophoresis and stained, and the stained spots were excised and digested with trypsin. The resulting peptide fragments were characterized by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Twenty-five proteins were identified as the proteins inside the virion and the acid-labile formyl group of an amino terminal proline residue of HIV-1(LAV-1) p24(gag) was determined by MALDI-TOF MS before and after weak-acid treatments (0.6 N hydrochloric acid) and confirmed by post-source decay (PSD) of the N-formylated N-terminal tryptic peptide (N-formylated Pro(1)-Arg(18)). The role of formylation has been unclear so far, but it is surmised that the acid-labile formylation of HIV-1(LAV-1) p24(gag) may play a critical role in the formation of the HIV-1 core for conferring HIV-1 infectivity.lld:pubmed
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pubmed-article:12051774pubmed:authorpubmed-author:TakahashiIchi...lld:pubmed
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pubmed-article:12051774pubmed:copyrightInfo(c) 2002 Elsevier Science (USA).lld:pubmed
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pubmed-article:12051774pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:12051774pubmed:articleTitleAcid-labile formylation of amino terminal proline of human immunodeficiency virus type 1 p24(gag) was found by proteomics using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.lld:pubmed
pubmed-article:12051774pubmed:affiliationDepartment of Biochemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-Honmachi, Kumamoto 862-0973, Japan.lld:pubmed
pubmed-article:12051774pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12051774pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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