Source:http://linkedlifedata.com/resource/pubmed/id/12051774
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
2002-6-7
|
pubmed:abstractText |
HIV-1(LAV-1) particles were collected by ultracentrifugation, treated with subtilisin, and then purified by Sepharose CL-4B column chromatography to remove microvesicles. The lysate of the purified human immunodeficiency virus type 1 (HIV-1) particles was subjected to two-dimensional (2D) gel electrophoresis and stained, and the stained spots were excised and digested with trypsin. The resulting peptide fragments were characterized by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Twenty-five proteins were identified as the proteins inside the virion and the acid-labile formyl group of an amino terminal proline residue of HIV-1(LAV-1) p24(gag) was determined by MALDI-TOF MS before and after weak-acid treatments (0.6 N hydrochloric acid) and confirmed by post-source decay (PSD) of the N-formylated N-terminal tryptic peptide (N-formylated Pro(1)-Arg(18)). The role of formylation has been unclear so far, but it is surmised that the acid-labile formylation of HIV-1(LAV-1) p24(gag) may play a critical role in the formation of the HIV-1 core for conferring HIV-1 infectivity.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Formic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Core Protein p24,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrochloric Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Proline,
http://linkedlifedata.com/resource/pubmed/chemical/Proteome,
http://linkedlifedata.com/resource/pubmed/chemical/Subtilisin,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
|
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0006-291X
|
pubmed:author | |
pubmed:copyrightInfo |
(c) 2002 Elsevier Science (USA).
|
pubmed:issnType |
Print
|
pubmed:day |
10
|
pubmed:volume |
293
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1107-13
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:12051774-Amino Acid Sequence,
pubmed-meshheading:12051774-Cell Line,
pubmed-meshheading:12051774-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:12051774-Formic Acids,
pubmed-meshheading:12051774-HIV Core Protein p24,
pubmed-meshheading:12051774-HIV-1,
pubmed-meshheading:12051774-Humans,
pubmed-meshheading:12051774-Hydrochloric Acid,
pubmed-meshheading:12051774-Molecular Sequence Data,
pubmed-meshheading:12051774-Proline,
pubmed-meshheading:12051774-Proteome,
pubmed-meshheading:12051774-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:12051774-Subtilisin,
pubmed-meshheading:12051774-Viral Proteins,
pubmed-meshheading:12051774-Virion
|
pubmed:year |
2002
|
pubmed:articleTitle |
Acid-labile formylation of amino terminal proline of human immunodeficiency virus type 1 p24(gag) was found by proteomics using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
|
pubmed:affiliation |
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-Honmachi, Kumamoto 862-0973, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|