Linked Life Data

12049754

Source:http://linkedlifedata.com/resource/pubmed/id/12049754

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-6-6
pubmed:abstractText
A novel tyrosinase biosensor has been developed for a subpicomolar detection of phenols, which is based on the immobilization of tyrosinase in a positively charged chitosan film on a glassy carbon electrode. It was found that chitosan cross-linked with (3-aminooryloxypropyl) dimethoxymethylsilane is beneficial for the immobilization of tyrosinase. The large microscopic surface area and porous morphology of chitosan matrix lead to high enzyme loading, and the enzyme entrapped in this matrix can retain its bioactivity and the positively charged surface of chitosan can also display a good anti-interference ability to the substances with positive charge. Hence, the resulting sensor offers a high-sensitivity (150 nA.nM(-1)) for the monitoring of phenols, and the detection limit is as low as 5.0 x 10(-11) M. Its response time is less than 2 s reaching 95% of the steady-state value. It may retain 75% of the activity for at least 70 days.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1567-5394
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Highly sensitive sensors based on the immobilization of tyrosinase in chitosan.
pubmed:affiliation
Department of Chemistry, Laboratory of Mesoscopic Materials and Chemistry, Institute of Analytical Science, Nanjing University, Nanjing 210093, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't