Linked Life Data

12006625

Source:http://linkedlifedata.com/resource/pubmed/id/12006625

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 11
pubmed:dateCreated
2002-5-13
pubmed:abstractText
Drosophila ELAV and human HuD are two neuronal RNA binding proteins that show remarkable sequence homology, yet differ in their respective documented roles in post-transcriptional regulation. ELAV regulates neural-specific alternative splicing of specific transcripts, and HuD stabilizes specific mRNAs that are otherwise unstable due to AU-rich elements (AREs) in their 3' untranslated region (UTR). AREs are major determinants of transcript stability in mammalian cells. The role of each of these proteins was investigated and compared, by ectopically expressing them in Drosophila imaginal wing disc cells, which lack endogenous expression of either protein. The effect of the ectopic expression of ELAV and HuD was assessed on two sets of green fluorescent protein reporter transgenes, which were all driven with a broadly expressing promoter. Each set consisted of three reporter transgenes: (1) with an uninterrupted open reading frame (ORF); (2) with a constitutively spliced intron inserted into the ORF; and (3) with the intron nASI whose splicing is regulated in neurons by ELAV, inserted into the ORF. The two sets differed from each other only in their 3'UTR: Heat-shock-protein-70Ab (Hsp70Ab) trailer with ARE-like characteristics or Actin 5C (Act5C) trailer. Our results show that: (1) both ectopically expressed ELAV and HuD can enhance expression of transgenes with the Hsp70Ab 3'UTR, but not of transgenes with Act5C 3'UTR; (2) this enhancement is accompanied by an increase in mRNA level; (3) only ELAV can induce neural-specific splicing of nASI; and (4) although HuD is localized primarily to the cytoplasm, ELAV is localized to both the cytoplasm and the nucleus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
115
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2413-21
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12006625-3' Untranslated Regions, pubmed-meshheading:12006625-Animals, pubmed-meshheading:12006625-Animals, Genetically Modified, pubmed-meshheading:12006625-Cell Differentiation, pubmed-meshheading:12006625-Choristoma, pubmed-meshheading:12006625-Drosophila melanogaster, pubmed-meshheading:12006625-Female, pubmed-meshheading:12006625-Gene Expression Regulation, Developmental, pubmed-meshheading:12006625-Genes, Regulator, pubmed-meshheading:12006625-Genes, Reporter, pubmed-meshheading:12006625-Green Fluorescent Proteins, pubmed-meshheading:12006625-HSP70 Heat-Shock Proteins, pubmed-meshheading:12006625-Hu Paraneoplastic Encephalomyelitis Antigens, pubmed-meshheading:12006625-Humans, pubmed-meshheading:12006625-Introns, pubmed-meshheading:12006625-Larva, pubmed-meshheading:12006625-Luminescent Proteins, pubmed-meshheading:12006625-Male, pubmed-meshheading:12006625-Nerve Tissue Proteins, pubmed-meshheading:12006625-Nervous System, pubmed-meshheading:12006625-Neurons, pubmed-meshheading:12006625-RNA, pubmed-meshheading:12006625-RNA Splicing, pubmed-meshheading:12006625-RNA-Binding Proteins, pubmed-meshheading:12006625-Ribonucleoproteins, pubmed-meshheading:12006625-Wing
pubmed:year
2002
pubmed:articleTitle
Ectopic expression of Drosophila ELAV and human HuD in Drosophila wing disc cells reveals functional distinctions and similarities.
pubmed:affiliation
Department of Biology and Center for Complex Systems, MS 008, Brandeis University, Waltham Massachusetts 02454, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.