Linked Life Data

12005070

Source:http://linkedlifedata.com/resource/pubmed/id/12005070

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-5-13
pubmed:abstractText
We previously reported that a fungal protein, p15, induces neurite outgrowth and differentiation of rat pheochromocytoma PC12 cells through the activation of the Ca2+ signaling pathway. We report here the secretory production of p15 in Aspergillus oryzae. Analysis of culture supernatant of A. oryzae transformed with the gene encoding the p15 precursor tagged with a hemagglutinin (HA) epitope demonstrated that the transformant secreted a protein with an apparent molecular mass of 17.5 kDa, which is a little larger than the expected size of mature p15-HA. By heat denaturation and ion exchange chromatography, p15-HA was easily purified from the culture supernatant with sufficient abundance. Although purified p15-HA was less active than the native p15 obtained from the culture broth of a producing fungal strain, it had neurite-inducing activity in PC12 cells in a dose-dependent manner, providing a system to study the action mechanism of p15.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0916-8451
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
674-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Functional expression in Aspergillus oryzae of p15, a protein with potent neurite-inducing activity in PC12 cells.
pubmed:affiliation
Department of Biotechnology, The University of Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't