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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6890
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pubmed:dateCreated |
2002-6-14
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pubmed:databankReference |
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pubmed:abstractText |
In bacteria, the binding of a single protein, the initiation factor sigma, to a multi-subunit RNA polymerase core enzyme results in the formation of a holoenzyme, the active form of RNA polymerase essential for transcription initiation. Here we report the crystal structure of a bacterial RNA polymerase holoenzyme from Thermus thermophilus at 2.6 A resolution. In the structure, two amino-terminal domains of the sigma subunit form a V-shaped structure near the opening of the upstream DNA-binding channel of the active site cleft. The carboxy-terminal domain of sigma is near the outlet of the RNA-exit channel, about 57 A from the N-terminal domains. The extended linker domain forms a hairpin protruding into the active site cleft, then stretching through the RNA-exit channel to connect the N- and C-terminal domains. The holoenzyme structure provides insight into the structural organization of transcription intermediate complexes and into the mechanism of transcription initiation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
417
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
712-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12000971-Amino Acid Sequence,
pubmed-meshheading:12000971-Binding Sites,
pubmed-meshheading:12000971-Catalytic Domain,
pubmed-meshheading:12000971-Crystallography, X-Ray,
pubmed-meshheading:12000971-DNA,
pubmed-meshheading:12000971-DNA-Binding Proteins,
pubmed-meshheading:12000971-DNA-Directed RNA Polymerases,
pubmed-meshheading:12000971-Holoenzymes,
pubmed-meshheading:12000971-Magnesium,
pubmed-meshheading:12000971-Models, Molecular,
pubmed-meshheading:12000971-Molecular Sequence Data,
pubmed-meshheading:12000971-Nucleic Acid Conformation,
pubmed-meshheading:12000971-Promoter Regions, Genetic,
pubmed-meshheading:12000971-Protein Structure, Secondary,
pubmed-meshheading:12000971-Protein Structure, Tertiary,
pubmed-meshheading:12000971-Sigma Factor,
pubmed-meshheading:12000971-Thermus thermophilus,
pubmed-meshheading:12000971-Zinc Fingers
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pubmed:year |
2002
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pubmed:articleTitle |
Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution.
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pubmed:affiliation |
Cellular Signaling Laboratory, RIKEN Harima Institute at Spring-8, 1-1-1 Kouto, Mikazuki-cho, Sayo, Hyogo 679-5148, Japan. dmitry@yumiyoshi.harima.riken.go.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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