|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0012940,
umls-concept:C0013138,
umls-concept:C0017337,
umls-concept:C0083956,
umls-concept:C0439855,
umls-concept:C0600508,
umls-concept:C1257890,
umls-concept:C1510941,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2002-4-25
|
|
pubmed:abstractText |
Regulatory DNA from the Drosophila gene engrailed causes silencing of a linked reporter gene (mini-white) in transgenic Drosophila. This silencing is strengthened in flies homozygous for the transgene and has been called "pairing-sensitive silencing." The pairing-sensitive silencing activities of a large fragment (2.6 kb) and a small subfragment (181 bp) were explored. Since pairing-sensitive silencing is often associated with Polycomb group response elements (PREs), we tested the activities of each of these engrailed fragments in a construct designed to detect PRE activity in embryos. Both fragments were found to behave as PREs in a bxd-Ubx-lacZ reporter construct, while the larger fragment showed additional silencing capabilities. Using the mini-white reporter gene, a 139-bp minimal pairing-sensitive element (PSE) was defined. DNA mobility-shift assays using Drosophila nuclear extracts suggested that there are eight protein-binding sites within this 139-bp element. Mutational analysis showed that at least five of these sites are important for pairing-sensitive silencing. One of the required sites is for the Polycomb group protein Pleiohomeotic and another is GAGAG, a sequence bound by the proteins GAGA factor and Pipsqueak. The identity of the other proteins is unknown. These data suggest a surprising degree of complexity in the DNA-binding proteins required for PSE function.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-10226011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-10322135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-10412979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-10500238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-10545463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-10644409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-10757791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-10757803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-10840724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-11124122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-11158316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-11389465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-11416132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-11420721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-11493537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-11493709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-11493925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-1571289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-1655566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-2573829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-2835286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-2850048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-6442354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-7498743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-7501470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-7504178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-7912192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-8005412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-8101825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-8632827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-8646782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-8825476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-9115424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-9258680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-9372908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-9468792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-9590167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-9651589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-9774480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11973310-9990850
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0016-6731
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
160
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1561-71
|
|
pubmed:dateRevised |
2011-5-4
|
|
pubmed:meshHeading |
pubmed-meshheading:11973310-Animals,
pubmed-meshheading:11973310-Animals, Genetically Modified,
pubmed-meshheading:11973310-Base Sequence,
pubmed-meshheading:11973310-Binding Sites,
pubmed-meshheading:11973310-DNA Mutational Analysis,
pubmed-meshheading:11973310-DNA-Binding Proteins,
pubmed-meshheading:11973310-Drosophila,
pubmed-meshheading:11973310-Gene Expression Regulation,
pubmed-meshheading:11973310-Gene Silencing,
pubmed-meshheading:11973310-Genes, Reporter,
pubmed-meshheading:11973310-Homeodomain Proteins,
pubmed-meshheading:11973310-Molecular Sequence Data,
pubmed-meshheading:11973310-Phenotype,
pubmed-meshheading:11973310-Response Elements,
pubmed-meshheading:11973310-Sequence Analysis, DNA,
pubmed-meshheading:11973310-Transcription Factors
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
A complex array of DNA-binding proteins required for pairing-sensitive silencing by a polycomb group response element from the Drosophila engrailed gene.
|
|
pubmed:affiliation |
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
|
|
pubmed:publicationType |
Journal Article
|
