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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-4-15
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pubmed:abstractText |
Transfection of keratinocytes with plasmid DNA leads to the loss of detectable DNA-binding activity of CCAAT displacement protein but not of Yin Yang 1, as monitored by electrophoretic mobility shift assay. This phenomenon was found to be attributable to the presence of plasmid DNA in the nuclear extracts prepared from transfected cells. Treatment of these nuclear extracts with DNase I restored the ability to monitor DNA-binding activity of CDP. This report documents a new pitfall associated with transfection.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CUX1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease I,
http://linkedlifedata.com/resource/pubmed/chemical/Erythroid-Specific DNA-Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/YY1 Transcription Factor
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1044-5498
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
109-13
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pubmed:dateRevised |
2008-9-13
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pubmed:meshHeading |
pubmed-meshheading:11953010-Binding, Competitive,
pubmed-meshheading:11953010-Cell Extracts,
pubmed-meshheading:11953010-Cell-Free System,
pubmed-meshheading:11953010-Cells, Cultured,
pubmed-meshheading:11953010-DNA,
pubmed-meshheading:11953010-DNA, Recombinant,
pubmed-meshheading:11953010-DNA-Binding Proteins,
pubmed-meshheading:11953010-Deoxyribonuclease I,
pubmed-meshheading:11953010-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:11953010-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:11953010-HeLa Cells,
pubmed-meshheading:11953010-Homeodomain Proteins,
pubmed-meshheading:11953010-Humans,
pubmed-meshheading:11953010-Keratinocytes,
pubmed-meshheading:11953010-Male,
pubmed-meshheading:11953010-Nuclear Proteins,
pubmed-meshheading:11953010-Plasmids,
pubmed-meshheading:11953010-Protein Binding,
pubmed-meshheading:11953010-Repressor Proteins,
pubmed-meshheading:11953010-Transcription, Genetic,
pubmed-meshheading:11953010-Transcription Factors,
pubmed-meshheading:11953010-Transfection,
pubmed-meshheading:11953010-YY1 Transcription Factor
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pubmed:year |
2002
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pubmed:articleTitle |
Transfection of keratinocytes abrogates detectable DNA-binding activity of CCAAT displacement protein.
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pubmed:affiliation |
Department of Microbiology and Immunology, Indiana University School of Medicine, and Walther Cancer Institute, Indianapolis, Indiana 46202-5120, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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