Linked Life Data

11918837

Source:http://linkedlifedata.com/resource/pubmed/id/11918837

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-3-28
pubmed:abstractText
Growth differentiation factor-9 (GDF-9), a member of the transforming growth factor-beta superfamily, is known to be expressed specifically in ovaries of various mammalian species and to be important for normal follicular development in mice. In the present study, a cDNA encoding for bovine GDF-9 was isolated and characterized, and expression of GDF-9 mRNA in ovarian oocytes and in-vitro-derived embryos was examined. Isolation of bovine GDF-9 was achieved using the polymerase chain reaction (PCR) with primers based on an ovine GDF-9 cDNA sequence. A 1385 bp cDNA encodes a deduced 453-amino acid sequence which contains the proregion (318 residues) and the mature protein (135 residues) portion. The deduced amino acid sequence of bovine GDF-9 is 98 and 93% identical to ovine GDF-9 and human GDF-9 in the mature portion of the molecule, respectively. Results from reverse transcription (RT)-PCR analysis detected bovine GDF-9 mRNA in preantral follicles (150-200 microm in diameter), early antral follicles (400-800 microm in diameter), and immature oocytes, whereas no detectable PCR signal was observed in cumulus/granulosa cells. In addition, bovine GDF-9 mRNA continued to be expressed in developing embryos up to the 8-cell stage, but was undetectable at the blastocyst stage. These findings give rise to new possibilities regarding an additional physiological role of GDF-9 in early embryonic development as well as in the development of follicles.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1520-4553
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3-10
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11918837-Amino Acid Sequence, pubmed-meshheading:11918837-Animals, pubmed-meshheading:11918837-Base Sequence, pubmed-meshheading:11918837-Blastocyst, pubmed-meshheading:11918837-Bone Morphogenetic Protein 15, pubmed-meshheading:11918837-Cattle, pubmed-meshheading:11918837-Cloning, Molecular, pubmed-meshheading:11918837-DNA, Complementary, pubmed-meshheading:11918837-Female, pubmed-meshheading:11918837-Fertilization in Vitro, pubmed-meshheading:11918837-Gene Expression Regulation, Developmental, pubmed-meshheading:11918837-Growth Differentiation Factor 9, pubmed-meshheading:11918837-Growth Substances, pubmed-meshheading:11918837-Humans, pubmed-meshheading:11918837-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:11918837-Mice, pubmed-meshheading:11918837-Molecular Sequence Data, pubmed-meshheading:11918837-Oocytes, pubmed-meshheading:11918837-Pregnancy, pubmed-meshheading:11918837-Sequence Alignment
pubmed:year
2001
pubmed:articleTitle
Molecular cloning of a cDNA encoding a bovine growth differentiation factor-9 (GDF-9) and expression of GDF-9 in bovine ovarian oocytes and in vitro-produced embryos.
pubmed:affiliation
Research Institute for the Functional Peptides, Yamagata, Japan. sendai@func-p.co.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't