|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
2002-3-21
|
|
pubmed:abstractText |
In human blood two monocyte populations can be distinguished, i.e., the CD14(++)CD16(-)DR(+) classical monocytes and the CD14(+)CD16(+)DR(++) proinflammatory monocytes that account for only 10% of all monocytes. We have studied TNF production in these two types of cells using three-color immunofluorescence and flow cytometry on whole peripheral blood samples stimulated with either LPS or with the bacterial lipopeptide S-(2,3-bis(palmitoyloxy)-(2-RS)-propyl)-N-palmitoyl-(R)-Cys-(S)-Ser-(S)-Lys(4)-OH,trihydrochloride (Pam3Cys). After stimulation with LPS the median fluorescence intensity for TNF protein was 3-fold higher in the proinflammatory monocytes when compared with the classical monocytes. After stimulation with Pam3Cys they almost exclusively responded showing 10-fold-higher levels of median fluorescence intensity for TNF protein. The median fluorescence intensity for Toll-like receptor 2 cell surface protein was found 2-fold higher on CD14(+)CD16(+)DR(++) monocytes, which may explain, in part, the higher Pam3Cys-induced TNF production by these cells. When analyzing secretion of TNF protein into the supernatant in PBMCs after depletion of CD16(+) monocytes we found a reduction of LPS-induced TNF by 28% but Pam3Cys-induced TNF was reduced by 64%. This indicates that the minor population of CD14(+)CD16(+) monocytes are major producers of TNF in human blood.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,3-bis(palmitoyloxy)-2-propyl-1-pal...,
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgG,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/TLR2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0022-1767
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
168
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3536-42
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11907116-Adjuvants, Immunologic,
pubmed-meshheading:11907116-Antigens, CD14,
pubmed-meshheading:11907116-Cells, Cultured,
pubmed-meshheading:11907116-Cysteine,
pubmed-meshheading:11907116-Drosophila Proteins,
pubmed-meshheading:11907116-Fluorescent Antibody Technique,
pubmed-meshheading:11907116-HLA-DR Antigens,
pubmed-meshheading:11907116-Humans,
pubmed-meshheading:11907116-Immunomagnetic Separation,
pubmed-meshheading:11907116-Immunophenotyping,
pubmed-meshheading:11907116-Inflammation,
pubmed-meshheading:11907116-Lipopolysaccharides,
pubmed-meshheading:11907116-Lipoproteins,
pubmed-meshheading:11907116-Membrane Glycoproteins,
pubmed-meshheading:11907116-Monocytes,
pubmed-meshheading:11907116-RNA, Messenger,
pubmed-meshheading:11907116-Receptors, Cell Surface,
pubmed-meshheading:11907116-Receptors, IgG,
pubmed-meshheading:11907116-Receptors, Immunologic,
pubmed-meshheading:11907116-Toll-Like Receptor 2,
pubmed-meshheading:11907116-Toll-Like Receptors,
pubmed-meshheading:11907116-Tumor Necrosis Factor-alpha
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
The proinflammatory CD14+CD16+DR++ monocytes are a major source of TNF.
|
|
pubmed:affiliation |
Institute for Immunology, University of Muenchen, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
