Linked Life Data

11868368

Source:http://linkedlifedata.com/resource/pubmed/id/11868368

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-2-28
pubmed:abstractText
We have previously shown that farnesylamine which acts as a farnesyltransferase inhibitor (FTI) induces apoptosis in human pancreatic cancer cells. Since the effect of FTI is usually known as "cytostatic", another apoptotic machinery of farnesylamine should be revealed in addition to the inhibition of farnesylation. Considering the chemical formula of farnesylamine, the "long-chain fatty amine (LFA)" structure may have a critical role in this mechanism. In this experiment, we examined the cytotoxic effect of LFA with alkyl-chain including oleylamine. Exposure of human pancreatic cancer cells to LFAs resulted in cell death, whereas short-chain fatty amine and oleylamine-related compounds without "amine" did not exert cytotoxicity. Oleylamine-induced cell death was confirmed as apoptosis by DNA laddering and caspase-dependent, but numerous cytoplasmic vacuoles, a typical feature of autophagy (type-2 cell death), were also observed. Preceding the apoptosis, strong and sustained activation of c-jun N-terminal kinase (JNK) and p38 was observed; caspase inhibitors did not attenuate activities of those kinases despite significant inhibition of apoptosis. Blockage of JNK activity by dominant-negative mutant completely abrogated the oleylamine-induced DNA laddering, but not autophagy. Furthermore, oleylamine decreased extracellular signal-regulated kinase (ERK) activity, probably through the activation of mitogen-activated protein kinase phosphatase-1. Taken together, LFA induces apoptosis through activation of JNK, p38 and caspase, accompanied with ERK inactivation, in human pancreatic cancer cells.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0367-6102
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17-29
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
[Oleylamine (long-chain fatty amine)-induced cell death through MAP kinase pathways in human pancreatic cancer cells].
pubmed:affiliation
Third Department of Internal Medicine, Asahikawa Medical College, Asahikawa 078-8510, Japan.
pubmed:publicationType
Journal Article, English Abstract