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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2002-4-29
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pubmed:databankReference |
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pubmed:abstractText |
Receptor-interacting protein (RIP) is a serine/threonine protein kinase that is critically involved in tumor necrosis factor receptor-1 (TNF-R1)-induced NF-kappa B activation. In a yeast two-hybrid screening for potential RIP-interacting proteins, we identified ZIN (zinc finger protein inhibiting NF-kappa B), a novel protein that specifically interacts with RIP. ZIN contains four RING-like zinc finger domains at the middle and a proline-rich domain at the C terminus. Overexpression of ZIN inhibits RIP-, IKK beta-, TNF-, and IL1-induced NF-kappa B activation in a dose-dependent manner in 293 cells. Domain mapping experiments indicate that the RING-like zinc finger domains of ZIN are required for its interaction with RIP and inhibition of RIP-mediated NF-kappa B activation. Overexpression of ZIN also potentiates RIP- and TNF-induced apoptosis. Moreover, immunofluorescent staining indicates that ZIN is a cytoplasmic protein and that it colocalizes with RIP. Our findings suggest that ZIN is an inhibitor of TNF- and IL1-induced NF-kappa B activation pathways.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RIPK1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor-Interacting Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15985-91
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11854271-Amino Acid Sequence,
pubmed-meshheading:11854271-Carrier Proteins,
pubmed-meshheading:11854271-Cell Line,
pubmed-meshheading:11854271-Humans,
pubmed-meshheading:11854271-Interleukin-1,
pubmed-meshheading:11854271-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:11854271-Molecular Sequence Data,
pubmed-meshheading:11854271-NF-kappa B,
pubmed-meshheading:11854271-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11854271-Proteins,
pubmed-meshheading:11854271-Receptor-Interacting Protein Serine-Threonine Kinases,
pubmed-meshheading:11854271-Recombinant Proteins,
pubmed-meshheading:11854271-Transfection,
pubmed-meshheading:11854271-Tumor Necrosis Factor-alpha,
pubmed-meshheading:11854271-Ubiquitin-Protein Ligases,
pubmed-meshheading:11854271-Zinc Fingers
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pubmed:year |
2002
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pubmed:articleTitle |
A novel zinc finger protein interacts with receptor-interacting protein (RIP) and inhibits tumor necrosis factor (TNF)- and IL1-induced NF-kappa B activation.
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pubmed:affiliation |
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, China.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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