Linked Life Data

11801667

Source:http://linkedlifedata.com/resource/pubmed/id/11801667

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-1-21
pubmed:abstractText
The reported requirement of functional Toll-like receptor (TLR)4 for resistance to Gram-negative pyelonephritis prompted us to localize the expression of TLR2 and TLR4 mRNA in the kidney at the cellular level by in situ hybridization. The majority of the constitutive TLR2 and TLR4 mRNA expression was found to be strategically located in the renal epithelial cells. Assuming that the TLR mRNA expression is representative of apical protein expression, this suggests that these cells are able to detect and react with bacteria present in the lumen of the tubules. To gain insight in the regulation of TLR expression during inflammation, we used a model for renal inflammation. Renal inflammation evoked by ischemia markedly enhanced synthesis of TLR2 and TLR4 mRNA in the distal tubular epithelium, the thin limb of Henle's loop, and collecting ducts. The increased renal TLR4 mRNA expression was associated with significant elevation of renal TLR4 protein expression as evaluated by Western blotting. Using RT-PCR, the enhanced TLR2 and TLR4 mRNA expression was shown to be completely dependent on the action of IFN-gamma and TNF-alpha. These results indicate a potential mechanism of increased immunosurveillance during inflammation at the site in which ascending bacteria enter the kidney tissue, i.e., the collecting ducts and the distal part of the nephron.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
168
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1286-93
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11801667-Animals, pubmed-meshheading:11801667-Disease Models, Animal, pubmed-meshheading:11801667-Drosophila Proteins, pubmed-meshheading:11801667-Epithelial Cells, pubmed-meshheading:11801667-Gene Expression Regulation, pubmed-meshheading:11801667-Inflammation, pubmed-meshheading:11801667-Interferon-gamma, pubmed-meshheading:11801667-Kidney, pubmed-meshheading:11801667-Male, pubmed-meshheading:11801667-Membrane Glycoproteins, pubmed-meshheading:11801667-Mice, pubmed-meshheading:11801667-RNA, Messenger, pubmed-meshheading:11801667-Receptors, Cell Surface, pubmed-meshheading:11801667-Reperfusion Injury, pubmed-meshheading:11801667-Toll-Like Receptor 2, pubmed-meshheading:11801667-Toll-Like Receptor 4, pubmed-meshheading:11801667-Toll-Like Receptors, pubmed-meshheading:11801667-Tumor Necrosis Factor-alpha, pubmed-meshheading:11801667-Up-Regulation
pubmed:year
2002
pubmed:articleTitle
In vivo expression of Toll-like receptor 2 and 4 by renal epithelial cells: IFN-gamma and TNF-alpha mediated up-regulation during inflammation.
pubmed:affiliation
Nutrition and Toxicology Research Institute Maastricht, Department of General Surgery, University of Maastricht, 6200 MD Maastricht, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't