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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-1-18
pubmed:databankReference
pubmed:abstractText
Epidemic-epizootic Venezuelan equine encephalitis (VEE) viruses (VEEV) have emerged repeatedly via convergent evolution from enzootic predecessors. However, previous sequence analyses have failed to identify common sets of nucleotide or amino acid substitutions associated with all emergence events. During 1993 and 1996, VEEV subtype IE epizootics occurred on the Pacific Coast of the states of Chiapas and Oaxaca in southern Mexico. Like other epizootic VEEV strains, when inoculated into guinea pigs and mice, the Mexican isolates were no more virulent than closely related enzootic strains, complicating genetic studies of VEE emergence. Complete genomic sequences of 4 of the Mexican strains were determined and compared to those of closely related enzootic subtype IE isolates from Guatemala. The epizootic viruses were less than 2% different at the nucleotide sequence level, and phylogenetic relationships confirmed that the equine-virulent Mexican strains probably evolved from enzootic progenitors on the Pacific Coast of Mexico or Guatemala. Of 35 amino acids that varied among the Guatemalan and Mexican isolates, only 8 were predicted phylogenetically to have accompanied the phenotypic change. One mutation at position 117 of the E2 envelope glycoprotein, involving replacement of Glu by Lys, resulted in a small-plaque phenotype characteristic of epizootic VEEV strains. Analysis of additional E2 sequences from representative enzootic and epizootic VEEV isolates implicated similar surface charge changes in the emergence of previous South American epizootic phenotypes, indicating that E2 mutations are probably important determinants of the equine-virulent phenotype and of VEE emergence. Maximum-likelihood analysis indicated that one change at E2 position 213 has been influenced by positive selection and convergent evolution of the epizootic phenotype.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-10196323, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-10348239, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-10466974, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-10548292, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-10623754, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-10756040, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-10790415, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-11021998, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-11390583, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-11425168, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-11504410, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-1259093, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-1448915, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-1469368, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-2146802, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-2524126, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-2873752, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-3184276, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-3893103, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-4380042, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-4397564, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-464194, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-4728689, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-507283, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-580289, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-7200732, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-7679745, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-7968923, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-8445371, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-851494, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-8623548, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-8709783, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-9230800, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-9261393, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-9452290, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-9684636, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-9696831, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-9696832, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-9811777, http://linkedlifedata.com/resource/pubmed/commentcorrection/11799167-9886206
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1718-30
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Positively charged amino acid substitutions in the e2 envelope glycoprotein are associated with the emergence of venezuelan equine encephalitis virus.
pubmed:affiliation
Center for Tropical Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.
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