|
pubmed:abstractText |
Cytomegalovirus (CMV) causes life-threatening infections in immunocompromised patients, especially those with acquired immunodeficiency or organ transplants. Therefore, early detection of CMV is important to guide the clinical management of actively infected patients. Because detection of replicative transcripts indicates that the virus is in the process of being replicated in the infected cell, we applied a novel, sensitive, ligation-dependent (LD)-PCR method to detect CMV immediate-early (IE) messenger RNA (mRNA), an indicator of viral replication.
|
|
pubmed:affiliation |
Molecular Pathology Laboratory, Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA.
|
