Source:http://linkedlifedata.com/resource/pubmed/id/11755918
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-12-28
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| pubmed:abstractText |
Interleukin-8 (IL-8) is one of cytokines detected at sites of inflammation and in macrophage-foam cells of atherosclerotic lesions. The expression of IL-8 gene can be induced in cholesterol loaded THP-1 macrophages by oxidized low density lipoprotein. We report for the first time that the expression of human IL-8 gene in THP-1 macrophages is upregulated in a time- and concentration-dependent manner by prostaglandin D2 metabolite 15-deoxy-delta12, 14 prostaglandin J2 (15d-PGJ2), which is a natural ligand for activation of peroxisome proliferator-activated receptor-gamma transcription factor. Studies to identify the signal transduction pathways involved showed that IL-8 upregulation-mediated by 15d-PGJ2 was markedly inhibited when the THP-1 macrophages were incubated with a highly selective and cell-permeable inhibitor of the mitogen-activated protein kinase (MAPK) signaling pathway, 2'-amino-3'-methoxyflavone (PD98059). This inhibition was concentration-dependent, suggesting that 15d-PGJ2 regulates the expression of IL-8 gene in THP-1 macrophages through a MAPK signaling pathway. In contrast, THP-1 macrophages when treated with pyrrolidine dithiocarbamate, an anti-oxidant and the selective inhibitor for nuclear factor kappaB, showed an enhanced 15d-PGJ2-mediated upregulation of IL-8 gene expression. The data presented in this report may contribute to unravel some of the mechanisms behind the inflammatory component of atherosclerosis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/15-deoxy-delta(12,14)-prostaglandin...,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Immunologic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin D2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0021-9150
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
160
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
11-20
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:11755918-Cholesterol, LDL,
pubmed-meshheading:11755918-Dose-Response Relationship, Drug,
pubmed-meshheading:11755918-Humans,
pubmed-meshheading:11755918-Immunologic Factors,
pubmed-meshheading:11755918-Interleukin-8,
pubmed-meshheading:11755918-Macrophages,
pubmed-meshheading:11755918-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11755918-Muscle, Smooth, Vascular,
pubmed-meshheading:11755918-NF-kappa B,
pubmed-meshheading:11755918-Prostaglandin D2,
pubmed-meshheading:11755918-RNA, Messenger,
pubmed-meshheading:11755918-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:11755918-Signal Transduction,
pubmed-meshheading:11755918-Transcription Factors,
pubmed-meshheading:11755918-Tretinoin,
pubmed-meshheading:11755918-Tumor Cells, Cultured,
pubmed-meshheading:11755918-Up-Regulation
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| pubmed:year |
2002
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| pubmed:articleTitle |
Upregulation of interleukin-8 expression by prostaglandin D2 metabolite 15-deoxy-delta12, 14 prostaglandin J2 (15d-PGJ2) in human THP-1 macrophages.
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| pubmed:affiliation |
Division of Endocrinology, Diabetes and Medical Genetics, Department of Medicine, Room 520, Strom Thurmond Biomedical Center, Medical University of South Carolina, 114 Doughty Street, Charleston, SC 29403-5729, USA. fuy@musc.edu
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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