Linked Life Data

11751191

Source:http://linkedlifedata.com/resource/pubmed/id/11751191

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2001-12-25
pubmed:abstractText
We wished to determine if the inflammatory cells surrounding the airway mucus-secreting glands in chronic bronchitis (CB) were associated with interleukin (IL)-4 and IL-5 mRNA expression and whether the CD8 T cell population expressed these cytokines. Digoxigenin-labeled IL-4 and IL-5 antisense RNA probes were used to detect gene expression in 11 asymptomic smokers (AS), 11 smokers with CB alone with normal lung function, and 10 smokers with chronic bronchitis and coexisting chronic obstructive pulmonary disease (CB+COPD; FEV(1)% of predicted of 43-77% and FEV(1)/ FVC of 51-68%). There were approximately three times as many IL-4 than IL-5 mRNA(+) cells. The highest number of IL-4 mRNA(+) cells were in the submucosal glands of the CB group with normal lung function (216/mm(2)), significantly higher than the values in either the AS (63/mm(2)) or the CB+COPD (87/mm(2)) groups, respectively (p < 0.01). There were similar group differences when the total numbers of inflammatory cells were compared. Accordingly, there was a positive correlation between the number of IL-4 mRNA(+) cells and the total number of inflammatory cells in both the subepithelium and glandular compartments (r = 0.60; p = 0.01 and r = 0.70; p = 0.02, respectively). There were no significant associations between the numbers of CD8(+) and IL-4 or IL-5 mRNA(+) cells. Of 1328 IL-4(+) and 1404 CD8(+) cells counted none was double labeled. Of 727 IL-5(+) and 1569 CD8(+) cells, none was double labeled. In contrast, as a positive control, 34% of tumor necrosis factor (TNF)-alpha(+) cells were also CD8(+) and 15% of CD8(+) cells were TNF-alpha positive. Thus, cells other than the CD8(+) phenotype produce IL-4 and IL-5 in CB. We conclude that there is increased inflammation and IL-4 gene expression in the mucus-secreting glands and the airway mucosa of smokers with bronchitis: both are lower in those with CB and coexisting COPD suggesting that airway inflammation in CB is reduced when airway obstruction develops.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1073-449X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
164
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2220-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11751191-Aged, pubmed-meshheading:11751191-Aged, 80 and over, pubmed-meshheading:11751191-Bronchi, pubmed-meshheading:11751191-Bronchitis, Chronic, pubmed-meshheading:11751191-CD8-Positive T-Lymphocytes, pubmed-meshheading:11751191-Epithelium, pubmed-meshheading:11751191-Exocrine Glands, pubmed-meshheading:11751191-Female, pubmed-meshheading:11751191-Gene Expression, pubmed-meshheading:11751191-Humans, pubmed-meshheading:11751191-Immunohistochemistry, pubmed-meshheading:11751191-In Situ Hybridization, pubmed-meshheading:11751191-Inflammation, pubmed-meshheading:11751191-Interleukin-4, pubmed-meshheading:11751191-Interleukin-5, pubmed-meshheading:11751191-Male, pubmed-meshheading:11751191-Middle Aged, pubmed-meshheading:11751191-Mucus, pubmed-meshheading:11751191-Pulmonary Disease, Chronic Obstructive, pubmed-meshheading:11751191-RNA, Messenger, pubmed-meshheading:11751191-Smoking
pubmed:year
2001
pubmed:articleTitle
Interleukin-4 and interleukin-5 gene expression and inflammation in the mucus-secreting glands and subepithelial tissue of smokers with chronic bronchitis. Lack of relationship with CD8(+) cells.
pubmed:affiliation
Department of Gene Therapy, Imperial College School of Medicine, London, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't