Linked Life Data

11748142

Source:http://linkedlifedata.com/resource/pubmed/id/11748142

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
2001-12-18
pubmed:databankReference
pubmed:abstractText
Mutations in the rolling pebbles (rols) gene result in severe defects in myoblast fusion. Muscle precursor cells are correctly determined, but myogenesis does not progress significantly beyond this point because recognition and/or cell adhesion between muscle precursor cells and fusion-competent myoblasts is disturbed. Molecular analysis of the rols genomic region reveals two variant transcripts of rols due to different transcription initiation sites, rols6 and rols7. rols6 mRNA is detectable mainly in the endoderm during differentiation as well as in malpighian tubules and in the epidermis. By contrast, rols7 expression is restricted to the mesoderm and later to progenitor descendants during somatic and pharyngeal muscle development. Transcription starts at the extended germ band stage when progenitor/founder cells are determined and persists until stage 13. The proteins encoded by the rols gene are 1670 (Rols6) and 1900 (Rols7) amino acids in length. Both forms contain an N-terminal RING-finger motif, nine ankyrin repeats and a TPR repeat eventually overlaid by a coiled-coil domain. The longer protein, Rols7, is characterized by 309 unique N-terminal amino acids, while Rols6 is distinguishable by 79 N-terminal amino acids. Expression of rols7 in muscle founder cells indicates a function of Rols7 in these cells. Transplantation assays of rols mutant mesodermal cells into wild-type embryos show that Rols is required in muscle precursor cells and is essential to recruit fusion-competent myoblasts for myotube formation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5061-73
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11748142-Amino Acid Sequence, pubmed-meshheading:11748142-Animals, pubmed-meshheading:11748142-Ankyrin Repeat, pubmed-meshheading:11748142-Cell Fusion, pubmed-meshheading:11748142-Chromosome Walking, pubmed-meshheading:11748142-Drosophila, pubmed-meshheading:11748142-Drosophila Proteins, pubmed-meshheading:11748142-Embryo, Nonmammalian, pubmed-meshheading:11748142-Genes, Insect, pubmed-meshheading:11748142-Giant Cells, pubmed-meshheading:11748142-Membrane Proteins, pubmed-meshheading:11748142-Molecular Sequence Data, pubmed-meshheading:11748142-Muscle Development, pubmed-meshheading:11748142-Muscle Fibers, Skeletal, pubmed-meshheading:11748142-Muscle Proteins, pubmed-meshheading:11748142-Muscles, pubmed-meshheading:11748142-Mutation, pubmed-meshheading:11748142-Nuclear Pore Complex Proteins, pubmed-meshheading:11748142-Pharyngeal Muscles, pubmed-meshheading:11748142-Proto-Oncogene Proteins, pubmed-meshheading:11748142-Stem Cells, pubmed-meshheading:11748142-Zinc Fingers
pubmed:year
2001
pubmed:articleTitle
rolling pebbles (rols) is required in Drosophila muscle precursors for recruitment of myoblasts for fusion.
pubmed:affiliation
Developmental Biology, Philipps-Universität Marburg, 35032 Marburg, Germany.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't