Source:http://linkedlifedata.com/resource/pubmed/id/11701740
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
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| pubmed:dateCreated |
2001-11-9
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| pubmed:abstractText |
IGF-I has been reported to play a role in regulating proliferation of human leiomyoma cells. There is, however, little evidence to suggest that IGF-I inhibits apoptosis in the leiomyoma cells. The present study was conducted to elucidate whether IGF-I affects apoptosis and Bcl-2 protein expression, an apoptosis-inhibiting gene product, in cultured leiomyoma cells. In addition, we examined the effect of IGF-I on proliferating cell nuclear antigen (PCNA) expression in cultured leiomyoma cells. Isolated human leiomyoma cells were subcultured in phenol red-free DMEM supplemented with 10% FBS for 120 h and then stepped down to serum-free conditions for an additional 72 h in the absence or presence of graded concentrations of IGF-I (1.0, 10, and 100 ng/ml). The effects of IGF-I on Bcl-2 protein and PCNA expression in cultured leiomyoma cells were assessed by Western immunoblot analysis and immunocytochemical staining, whereas the effects of IGF-I on the cell viability and apoptosis of the cultured cells were determined by 3-(4,5-dimethylatriazol-2-yl)-2,5diphenyltetrasodium bromide (MTT) assay and terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end labeling assay, respectively. Immunocytochemical staining demonstrated that IGF-I treatment resulted in the increase in PCNA labeling index in cultured leiomyoma cells in a dose-dependent manner. Immunoblot analysis of proteins extracted from the cultured leiomyoma cells revealed that the addition of IGF-I (10 and 100 ng/ml) significantly increased the expression of 35-kDa immunoreactive PCNA and 26-kDa Bcl-2 protein, compared with those in control cultures. Cell survival and proliferation of cultured leiomyoma cells, assessed by MTT assay, was significantly augmented by IGF-I treatment, compared with those of control cultures. Terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end labeling assay showed that the apoptosis-positive rate of leiomyoma cells treated with IGF-I was significantly decreased, compared with that in control cultures. The present results suggest that IGF-I plays crucial roles in leiomyoma cell growth, not only in promoting the proliferative potential by up-regulation of PCNA expression but also in down-regulating apoptosis by up-regulation of Bcl-2 protein expression in leiomyoma cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0021-972X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
86
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
5593-9
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11701740-Adult,
pubmed-meshheading:11701740-Apoptosis,
pubmed-meshheading:11701740-Blotting, Western,
pubmed-meshheading:11701740-Cell Division,
pubmed-meshheading:11701740-Cell Survival,
pubmed-meshheading:11701740-Female,
pubmed-meshheading:11701740-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:11701740-Humans,
pubmed-meshheading:11701740-Immunohistochemistry,
pubmed-meshheading:11701740-In Situ Nick-End Labeling,
pubmed-meshheading:11701740-Insulin-Like Growth Factor I,
pubmed-meshheading:11701740-Leiomyoma,
pubmed-meshheading:11701740-Middle Aged,
pubmed-meshheading:11701740-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:11701740-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:11701740-Tumor Cells, Cultured,
pubmed-meshheading:11701740-Up-Regulation,
pubmed-meshheading:11701740-Uterine Neoplasms
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| pubmed:year |
2001
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| pubmed:articleTitle |
Up-regulation by IGF-I of proliferating cell nuclear antigen and Bcl-2 protein expression in human uterine leiomyoma cells.
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| pubmed:affiliation |
Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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