Source:http://linkedlifedata.com/resource/pubmed/id/11691804
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
21
|
| pubmed:dateCreated |
2001-11-5
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| pubmed:abstractText |
The etiological role of human papillomaviruses (HPV) in cervical and other cancers suggests that therapeutic vaccines directed against requisite viral antigens may eradicate tumors or their precursors. A Venezuelan equine encephalitis (VEE) alphavirus vector delivering the HPV16 E7 RNA was evaluated for antitumor efficacy using a murine E7+ tumor model. Vaccination with VEE replicon particles expressing E7 (E7-VRP) induced class I-restricted CD8+ T-cell responses as determined by IFN-gamma enzyme-linked immunospot (ELISPOT), tetramer, and cytotoxicity assays. E7-VRP vaccination prevented tumor development in all of the mice and effectively eliminated 7-day established tumors in 67% of tumor-bearing mice. The induction of protective T-cell responses was dependent on CD8+, but not CD4+ T cells. Long-lasting T-cell memory responses developed in E7-VRP-vaccinated mice as determined by complete protection from tumor challenge 3 months after the final vaccination. These promising results highlight the potent CD8+ T-cell-mediated antitumor effects elicited by VEE replicon-based vectors and support their further development toward clinical testing against cervical intraepithelial neoplasia or carcinoma.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Papillomavirus E7 Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/oncogene protein E7, Human...
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
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| pubmed:issn |
0008-5472
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
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| pubmed:volume |
61
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
7861-7
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:11691804-Animals,
pubmed-meshheading:11691804-Cancer Vaccines,
pubmed-meshheading:11691804-Encephalitis Virus, Venezuelan Equine,
pubmed-meshheading:11691804-Female,
pubmed-meshheading:11691804-Gene Therapy,
pubmed-meshheading:11691804-Genetic Vectors,
pubmed-meshheading:11691804-Immunologic Memory,
pubmed-meshheading:11691804-Interferon-gamma,
pubmed-meshheading:11691804-Mice,
pubmed-meshheading:11691804-Mice, Inbred C57BL,
pubmed-meshheading:11691804-Mice, Knockout,
pubmed-meshheading:11691804-Neoplasms, Experimental,
pubmed-meshheading:11691804-Oncogene Proteins, Viral,
pubmed-meshheading:11691804-Papillomavirus E7 Proteins,
pubmed-meshheading:11691804-RNA, Viral,
pubmed-meshheading:11691804-Replicon,
pubmed-meshheading:11691804-T-Lymphocyte Subsets,
pubmed-meshheading:11691804-Vaccines, Synthetic
|
| pubmed:year |
2001
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| pubmed:articleTitle |
Eradication of established tumors by vaccination with Venezuelan equine encephalitis virus replicon particles delivering human papillomavirus 16 E7 RNA.
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| pubmed:affiliation |
Cardinal Bernardin Cancer Center, Loyola University-Chicago, Maywood, Illinois 60153, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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