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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
52
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pubmed:dateCreated |
2001-12-25
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pubmed:abstractText |
We have used a combination of fluorescence anisotropy spectroscopy and fluorescence-based native gel electrophoresis methods to examine the effects of the transcription factor IID-specific subunit TAF130p (TAF145p) upon the TATA box DNA binding properties of TATA box-binding protein (TBP). Purified full-length recombinant TAF130p decreases TBP-TATA DNA complex formation at equilibrium by competing directly with DNA for binding to TBP. Interestingly, we have found that full-length TAF130p is capable of binding multiple molecules of TBP with nanomolar binding affinity. The biological implications of these findings are discussed.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TAF1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/TATA-Binding Protein Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/TATA-Box Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor TFIID,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
49100-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11677244-DNA,
pubmed-meshheading:11677244-DNA-Binding Proteins,
pubmed-meshheading:11677244-Electrophoresis,
pubmed-meshheading:11677244-Fluorescence Polarization,
pubmed-meshheading:11677244-Macromolecular Substances,
pubmed-meshheading:11677244-Protein Binding,
pubmed-meshheading:11677244-Protein Subunits,
pubmed-meshheading:11677244-Recombinant Proteins,
pubmed-meshheading:11677244-Saccharomyces cerevisiae,
pubmed-meshheading:11677244-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:11677244-Spectrometry, Fluorescence,
pubmed-meshheading:11677244-TATA Box,
pubmed-meshheading:11677244-TATA-Binding Protein Associated Factors,
pubmed-meshheading:11677244-TATA-Box Binding Protein,
pubmed-meshheading:11677244-Transcription Factor TFIID,
pubmed-meshheading:11677244-Transcription Factors
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pubmed:year |
2001
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pubmed:articleTitle |
Fluorescence-based analyses of the effects of full-length recombinant TAF130p on the interaction of TATA box-binding protein with TATA box DNA.
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pubmed:affiliation |
Department of Molecular Physiology and Biophysics, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232-0615, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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