Source:http://linkedlifedata.com/resource/pubmed/id/11668390
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
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| pubmed:dateCreated |
2001-10-22
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| pubmed:abstractText |
Microarray analysis allows the screening of thousands of identifiable genes in a single experiment. The challenge of this approach is to combine the new technology with established genetic tools to associate genes with specific biological function. In this study we have designed a screen to identify imprinted genes from mice with uniparental duplications of proximal Chromosomes (Chrs) 7 and 11, using microarray analysis. By comparing the expression patterns in embryonic and newborn tissues of maternally versus paternally inherited proximal Chrs 7 and 11, we have correctly identified four out of five known imprinted genes represented on a microarray. We have additionally identified two novel imprinted candidate genes as well as a differentially expressed clone that is a potential downstream target. Interpretation of the microarray data requires careful preparation of age- and strain-matched samples and attention to detail in tissue dissection technique.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
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| pubmed:issn |
0938-8990
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
12
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
758-64
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11668390-Alleles,
pubmed-meshheading:11668390-Animals,
pubmed-meshheading:11668390-Base Sequence,
pubmed-meshheading:11668390-Brain,
pubmed-meshheading:11668390-Chromosome Aberrations,
pubmed-meshheading:11668390-Chromosomes,
pubmed-meshheading:11668390-Crosses, Genetic,
pubmed-meshheading:11668390-Female,
pubmed-meshheading:11668390-Gene Expression Profiling,
pubmed-meshheading:11668390-Gene Expression Regulation, Developmental,
pubmed-meshheading:11668390-Genomic Imprinting,
pubmed-meshheading:11668390-Male,
pubmed-meshheading:11668390-Mice,
pubmed-meshheading:11668390-Mice, Inbred C57BL,
pubmed-meshheading:11668390-Mice, Inbred Strains,
pubmed-meshheading:11668390-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:11668390-RNA, Messenger,
pubmed-meshheading:11668390-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11668390-Translocation, Genetic
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| pubmed:year |
2001
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| pubmed:articleTitle |
Microarray expression profiling of tissues from mice with uniparental duplications of chromosomes 7 and 11 to identify imprinted genes.
|
| pubmed:affiliation |
Division of Human Genetics, The Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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