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rdf:type |
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lifeskim:mentions |
umls-concept:C0026336,
umls-concept:C0026339,
umls-concept:C0040300,
umls-concept:C0205314,
umls-concept:C0445750,
umls-concept:C0449943,
umls-concept:C0450363,
umls-concept:C0679622,
umls-concept:C0699748,
umls-concept:C1706853,
umls-concept:C1879748,
umls-concept:C2603343
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pubmed:issue |
11
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pubmed:dateCreated |
2001-10-12
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pubmed:abstractText |
The lack of readily available experimental systems has limited knowledge pertaining to the development of Salmonella-induced gastroenteritis and diarrheal disease in humans. We used a novel low-shear stress cell culture system developed at the National Aeronautics and Space Administration in conjunction with cultivation of three-dimensional (3-D) aggregates of human intestinal tissue to study the infectivity of Salmonella enterica serovar Typhimurium for human intestinal epithelium. Immunohistochemical characterization and microscopic analysis of 3-D aggregates of the human intestinal epithelial cell line Int-407 revealed that the 3-D cells more accurately modeled human in vivo differentiated tissues than did conventional monolayer cultures of the same cells. Results from infectivity studies showed that Salmonella established infection of the 3-D cells in a much different manner than that observed for monolayers. Following the same time course of infection with Salmonella, 3-D Int-407 cells displayed minimal loss of structural integrity compared to that of Int-407 monolayers. Furthermore, Salmonella exhibited significantly lower abilities to adhere to, invade, and induce apoptosis of 3-D Int-407 cells than it did for infected Int-407 monolayers. Analysis of cytokine expression profiles of 3-D Int-407 cells and monolayers following infection with Salmonella revealed significant differences in expression of interleukin 1alpha (IL-1alpha), IL-1beta, IL-6, IL-1Ra, and tumor necrosis factor alpha mRNAs between the two cultures. In addition, uninfected 3-D Int-407 cells constitutively expressed higher levels of transforming growth factor beta1 mRNA and prostaglandin E2 than did uninfected Int-407 monolayers. By more accurately modeling many aspects of human in vivo tissues, the 3-D intestinal cell model generated in this study offers a novel approach for studying microbial infectivity from the perspective of the host-pathogen interaction.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-10398673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-10456944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-10844685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-11050248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-11399642,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-13475808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-1616701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-172399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-3021848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-3316027,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-7971958,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-8642291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-8790417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-8864120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-8885278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-8903327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-8970727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-9024292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-9125566,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-9194702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-9218506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-9364916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-9552004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-9573122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-9701241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-9819367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11598087-9916113
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pubmed:keyword |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0019-9567
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pubmed:author |
pubmed-author:BuchananK LKL,
pubmed-author:ClarkeM SMS,
pubmed-author:EmamiKK,
pubmed-author:GoodwinT JTJ,
pubmed-author:HammonsCC,
pubmed-author:LeBlancC LCL,
pubmed-author:NickersonC ACA,
pubmed-author:OttC MCM,
pubmed-author:PiersonD LDL,
pubmed-author:RamamurthiBB,
pubmed-author:TerlongeJJ,
pubmed-author:UickerW CWC,
pubmed-author:VanderburgC RCR
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7106-20
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11598087-Apoptosis,
pubmed-meshheading:11598087-Bacterial Adhesion,
pubmed-meshheading:11598087-Cell Line,
pubmed-meshheading:11598087-Cytokines,
pubmed-meshheading:11598087-Dinoprostone,
pubmed-meshheading:11598087-Humans,
pubmed-meshheading:11598087-Immunoenzyme Techniques,
pubmed-meshheading:11598087-Intestinal Mucosa,
pubmed-meshheading:11598087-Microscopy, Electron,
pubmed-meshheading:11598087-Models, Biological,
pubmed-meshheading:11598087-Salmonella typhimurium
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pubmed:year |
2001
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pubmed:articleTitle |
Three-dimensional tissue assemblies: novel models for the study of Salmonella enterica serovar Typhimurium pathogenesis.
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pubmed:affiliation |
Program in Molecular Pathogenesis and Immunity, Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA. cnicker@tulane.edu
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