Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
50
pubmed:dateCreated
2001-12-12
pubmed:abstractText
The cohesin multiprotein complex containing SMC1, SMC3, Scc3 (SA), and Scc1 (Rad21) is required for sister chromatid cohesion in eukaryotes. Although metazoan cohesin associates with chromosomes and was shown to function in the establishment of sister chromatid cohesion during interphase, the majority of cohesin was found to be off chromosomes and reside in the cytoplasm in metaphase. Despite its dissociation from chromosomes, however, microinjection of an antibody against human SMC1 led to disorganization of the metaphase plate and cell cycle arrest, indicating that human cohesin still plays an important role in metaphase. To address the mitotic function of human cohesin, the subcellular localization of cohesin components was reexamined in human cells. Interestingly, we found that cohesin localizes to the spindle poles during mitosis and interacts with NuMA, a spindle pole-associated factor required for mitotic spindle organization. The interaction with NuMA persists during interphase. Similar to NuMA, a significant amount of cohesin was found to associate with the nuclear matrix. Furthermore, in the absence of cohesin, mitotic spindle asters failed to form in vitro. Our results raise the intriguing possibility that in addition to its well demonstrated function in sister chromatid cohesion, cohesin may be involved in spindle assembly during mitosis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47575-82
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11590136-Animals, pubmed-meshheading:11590136-Antigens, Nuclear, pubmed-meshheading:11590136-Blotting, Western, pubmed-meshheading:11590136-Cell Cycle, pubmed-meshheading:11590136-Cell Cycle Proteins, pubmed-meshheading:11590136-Cell Nucleus, pubmed-meshheading:11590136-Chromatids, pubmed-meshheading:11590136-Chromosomal Proteins, Non-Histone, pubmed-meshheading:11590136-Chromosomes, pubmed-meshheading:11590136-Dimerization, pubmed-meshheading:11590136-Fungal Proteins, pubmed-meshheading:11590136-HeLa Cells, pubmed-meshheading:11590136-Humans, pubmed-meshheading:11590136-Interphase, pubmed-meshheading:11590136-Metaphase, pubmed-meshheading:11590136-Microscopy, Fluorescence, pubmed-meshheading:11590136-Mitosis, pubmed-meshheading:11590136-Mitotic Spindle Apparatus, pubmed-meshheading:11590136-Nuclear Matrix-Associated Proteins, pubmed-meshheading:11590136-Nuclear Proteins, pubmed-meshheading:11590136-Precipitin Tests, pubmed-meshheading:11590136-Protein Binding, pubmed-meshheading:11590136-Time Factors, pubmed-meshheading:11590136-Ultracentrifugation
pubmed:year
2001
pubmed:articleTitle
A potential role for human cohesin in mitotic spindle aster assembly.
pubmed:affiliation
Department of Biological Chemistry, College of Medicine, University of California, Irvine, CA 92697-1700, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't