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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
46
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pubmed:dateCreated |
2001-11-12
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pubmed:abstractText |
Although a major effect of p21, a cyclin-dependent kinase inhibitor, is considered to be exerted during G(1) phase of the cell cycle, p21 gene knock-out studies suggested its involvement in G(2)/M checkpoint as well. Here we demonstrate evidence that p21 is required for the cell cycle arrest at G(2) upon DNA damage. We found that expression of wild-type p21 (p21(WT)), not mutant p21 (p21(PCNA-)) lacking the interaction with proliferating cell nuclear antigen (PCNA), caused G(2) cell cycle arrest in p53-deficient DLD1 colon cancer cell line after the DNA damage by treatment with cis-diamminedichloroplatinum (II). We also found that p21(WT) was associated with Cdc2/cyclin B1 together with PCNA. Furthermore, coimmunoprecipitation experiments revealed that PCNA interacted with Cdc25C at the G(2)/M transition, and this interaction was abolished when p21(WT) was expressed presumably due to the competition between p21(WT) and Cdc25C in the binding to PCNA. These findings suggest that p21 plays a regulatory role in the maintenance of cell cycle arrest at G(2) by blocking the interaction of Cdc25C with PCNA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
42971-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11559705-Amino Acid Sequence,
pubmed-meshheading:11559705-Antineoplastic Agents,
pubmed-meshheading:11559705-Binding, Competitive,
pubmed-meshheading:11559705-Blotting, Western,
pubmed-meshheading:11559705-Cisplatin,
pubmed-meshheading:11559705-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:11559705-Cyclins,
pubmed-meshheading:11559705-DNA Damage,
pubmed-meshheading:11559705-Flow Cytometry,
pubmed-meshheading:11559705-G2 Phase,
pubmed-meshheading:11559705-Genes, p53,
pubmed-meshheading:11559705-Humans,
pubmed-meshheading:11559705-Mitosis,
pubmed-meshheading:11559705-Molecular Sequence Data,
pubmed-meshheading:11559705-Mutation,
pubmed-meshheading:11559705-Phosphorylation,
pubmed-meshheading:11559705-Precipitin Tests,
pubmed-meshheading:11559705-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:11559705-Protein Binding,
pubmed-meshheading:11559705-Time Factors,
pubmed-meshheading:11559705-Tumor Cells, Cultured
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pubmed:year |
2001
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pubmed:articleTitle |
Involvement of the interaction between p21 and proliferating cell nuclear antigen for the maintenance of G2/M arrest after DNA damage.
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pubmed:affiliation |
Department of Molecular Genetics and First Department of Internal Medicine, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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