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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-9-13
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pubmed:abstractText |
We cloned a cDNA for Drosophila mitochondrial transcription factor A (D-mtTFA) and characterized the recombinant protein. In Drosophila Kc cells, D-mtTFA was localized in the mitochondria, but not in the nucleus. By repetitive precipitation with His-tag and PCR amplification, the consensus nucleotide sequence for D-mtTFA-binding was determined to be 5'-TTATC/G. The binding sequence was found to be clustered in the A + T region of mitochondrial DNA which is suggested to be a replication origin and promoter region for light strand and heavy strand. We found a DNA replication-related element (DRE)-like sequence located upstream of the transcription initiation site of the D-mtTFA gene and obtained results indicating that DRE-binding factor (DREF) can bind to the DRE-like sequence of the D-mtTFA gene. The data suggest that transcription of the D-mtTFA gene is under control of the DRE/DREF regulatory system. Based on these results, the functions of D-mtTFA were discussed in relation to mitochondrial biogenesis of Drosophila melanogaster.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
287
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
474-83
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:11554753-Animals,
pubmed-meshheading:11554753-Base Sequence,
pubmed-meshheading:11554753-Cell Division,
pubmed-meshheading:11554753-Cells, Cultured,
pubmed-meshheading:11554753-Cloning, Molecular,
pubmed-meshheading:11554753-Consensus Sequence,
pubmed-meshheading:11554753-DNA, Complementary,
pubmed-meshheading:11554753-DNA-Binding Proteins,
pubmed-meshheading:11554753-Drosophila melanogaster,
pubmed-meshheading:11554753-Gene Expression Regulation,
pubmed-meshheading:11554753-Humans,
pubmed-meshheading:11554753-Mitochondria,
pubmed-meshheading:11554753-Mitochondrial Proteins,
pubmed-meshheading:11554753-Molecular Sequence Data,
pubmed-meshheading:11554753-Molecular Weight,
pubmed-meshheading:11554753-Nuclear Proteins,
pubmed-meshheading:11554753-Subcellular Fractions,
pubmed-meshheading:11554753-Transcription Factors
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pubmed:year |
2001
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pubmed:articleTitle |
Drosophila mitochondrial transcription factor A: characterization of its cDNA and expression pattern during development.
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pubmed:affiliation |
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan.
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pubmed:publicationType |
Journal Article
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