Linked Life Data

11549417

Source:http://linkedlifedata.com/resource/pubmed/id/11549417

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-9-10
pubmed:abstractText
The recognition of a pathogen or a vaccine antigen formulation by cells in the innate immune system leads to production of proinflammatory cytokines, which will determine the ensuing acquired immune response quantitatively and qualitatively. Tumour necrosis factor (TNF)-alpha, interleukin (IL)-1 and IL-6 are the first set of cytokines produced upon such an encounter, which have roles both in protective immunity and immunopathogenesis evident with respiratory syncytial virus (RSV). RSV antigens in different physical adjuvant-vaccine formulations were analysed for their capacity to provoke cultured murine peritoneal cells to produce these three proinflammatory cytokines. RSV immunostimulating complex (ISCOM), i.e. both antigen and adjuvant are incorporated in the same particle, induced high levels of IL-1alpha being of the same magnitude or higher than those of live RSV and lipopolysaccharide (LPS). Live virus and LPS induced higher levels of IL-6 and TNF-alpha than ISCOM and so did non-adjuvanted UV-inactivated RSV but only at high doses. ISCOM-Matrix, i.e. ISCOM without antigens, admixed as a separate entity to inactivated RSV, downregulated or blocked the cytokine response to the inactivated RSV in contrast to ISCOM. Kinetic studies showed that ISCOM induced cytokine production first detected at hours 1, 2, 4 for TNF-alpha, IL-6 and IL-1alpha respectively, which was earlier than for the other antigen formulations containing corresponding doses of antigen and/or Quillaja adjuvant. Peak values for production of TNF-alpha and IL-6 were at 8 h and for IL-1alpha at 72 h following stimulation with ISCOM. The delayed appearance of IL-1alpha may reflect the cell-bound nature of this cytokine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0928-8244
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
105-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11549417-Adjuvants, Immunologic, pubmed-meshheading:11549417-Animals, pubmed-meshheading:11549417-Cell Line, pubmed-meshheading:11549417-Cell Survival, pubmed-meshheading:11549417-Cells, Cultured, pubmed-meshheading:11549417-Cytokines, pubmed-meshheading:11549417-Dose-Response Relationship, Immunologic, pubmed-meshheading:11549417-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:11549417-Female, pubmed-meshheading:11549417-Interleukin-1, pubmed-meshheading:11549417-Interleukin-6, pubmed-meshheading:11549417-Kinetics, pubmed-meshheading:11549417-Lipopolysaccharides, pubmed-meshheading:11549417-Macrophages, Peritoneal, pubmed-meshheading:11549417-Mice, pubmed-meshheading:11549417-Mice, Inbred BALB C, pubmed-meshheading:11549417-Respiratory Syncytial Virus Vaccines, pubmed-meshheading:11549417-Respiratory Syncytial Viruses, pubmed-meshheading:11549417-Saponins, pubmed-meshheading:11549417-Tumor Necrosis Factor-alpha
pubmed:year
2001
pubmed:articleTitle
Different respiratory syncytial virus and Quillaja saponin formulations induce murine peritoneal cells to express different proinflammatory cytokine profiles.
pubmed:affiliation
Faculty of Veterinary Medicine, Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't